（毕业设计论文）《芽孢杆菌D-13产淀粉酶条件的优化》.docVIP

PAGE 海 南 大 学 毕 业 论 文（设计） 题 目： 芽孢杆菌D-13产淀粉酶条件的优化 学 号： B0712027 姓 名： 年 级： 2007 级 学 院： 环境与植物保护学院 系 别： 农产品质量与安全系 专 业： 农产品质量与安全 指导教师： 完成日期： 2010年 10月 20 日 摘 要 淀粉酶是能水解淀粉和糖原的一类酶，是一种用途极广的生物催化剂，可应用于面包制作、淀粉的糖化和液化、纺织品脱浆、造纸业、清洁剂工业、化学和临床医学分析和制药业等。 本实验对一株分离自土壤的芽孢杆菌D-13产淀粉酶的条件进行了研究，利用单因素法对可溶性淀粉、氮源、酵母膏、NaCl、初始PH值及产酶时间7个因子进行了优化，得到芽孢杆菌D-13产淀粉酶的最适培养条件是：蛋白胨1.0%、可溶性淀粉3.5%、酵母膏0.4%、NaCl 0.3%、初始pH值为8.0，最佳培养时间为24小时，在最优条件下，酶活达到439.7U/mL。 关键词：芽孢杆菌 淀粉酶 优化 Abstract Amylase can hydrolysis starch and glycogen, which is usely extensively in biological catalyst,bread manufacture, starch saccharification and liquefaction, paper making, textile, detergent industrial, chemical and clinical medical and pharmaceutical analysis etc. This experiment was mainly on optimization of amylase production from Bacillus sp. D - 13. The factors of soluble starch, nitrogen source, yeast extract, NaCl, initial pH and time were optimized. The optimal cultivation condition was: peptone 1.0%, soluble starch 3.5%, yeast extract 0.4%, NaCl 0.3%, initial pH 8.0. Under the optimal conditions, enzyme production was up to 439.7 U/mL. Keywords: Bacillus sp. Amylase optimization 目 录 前言·······························································5 1 材料与方法·······················································6 1.1材料····························································6 1.1.1菌株来源·······················································6 1.1.2培养基·························································6 1.1.3相关试剂的配制·················································6 1.1.4主要的实验药品与仪器···········································6 1.2 实验方