清胰汤对重症急性胰腺炎大鼠白介素8白介素10影响.docVIP

清胰汤对重症急性胰腺炎大鼠白介素8白介素10影响 　　摘 要：目的：从实验上探讨清胰汤对重症急性胰腺炎（SAP）白介素-8（IL-8）、白介素-10（IL-10）的影响。方法：将60只大鼠随机分为随机分为正常对照组（假手术组）、模型组（重症急性胰腺炎组）、治疗组（清胰汤组），每组20只，造模后24h测定血淀粉酶、IL-8及IL-10，切取相同部位的胰腺组织观察组织病理学改变。结果：模型组及清胰汤组大鼠血清淀粉酶、IL-8及IL-10的浓度均显著高于对照组，清胰汤组大鼠血清淀粉酶、IL-8的浓度显著低于模型组；清胰汤组胰腺局部炎症反应较模型组减轻，间质水肿减轻，腺泡坏死减少，粒细胞浸润减少。结论：IL-8及IL-10在重症急性胰腺炎的发生发展中起一定作用，清胰汤治疗组IL-8及IL-10水平下降，表明清胰汤可能通过下调重症急性胰腺炎大鼠的IL-8及IL-10途径起治疗作用。? 　　关键词： 重症急性胰腺炎；清胰汤；白介素-8；白介素-10 　　中图分类号：R285.5文献标识码：A 　　文章编号：1673-7717(2008)05-1039-03? 　　Effect of Qingyi Decoction on Changes of IL-8 and IL-10 ?in Rat Serum with Severe Acute Pancreatitis 　　?? 　　JIN Mu?dan,ZHOU Zhi?lin,FU Zhi?quan? 　　(Gastroenterology Department,The Third Peoples Hospital of Hangzhou，Hangzhou 310009,Zhejiang,China 　　)? 　　Abstract:Objective:To evaluate the effect of Qingyi decoction on the changes of IL-8 and IL-10 in rat serum with severe acute pancreatitis (SAP). Methods:60 Wistar rats were randomly divided into normal control group (Sham operation group),model group(SAP group) and Therapeutic group (Qingyi decoction group). There were 20 rats in each group.Qingyi decoction was administered orally 30 minutes before model induction，12 hours and 24 hours after model induction. Amy,IL-8 and IL-10 in serum and pancreas tissue were determined and the pathological study was performed as well. Results:The levels of IL-8 and IL-10 in serum SAP and Qingyi decoction group were significantly higher than those in normal control group.The levels of Amy，IL-8 and IL-10 in Qingyi decoction group were significantly lower in SAP group. In Qingyi decoction group pancreas injury was markedly reduced. Conclusion:The results suggest IL-8 and IL-10 play an important role in severe acute pancreatitis.Qingyi decoction probably has protective effects on IL-8 and IL-10 pathways． 　　Keywords:severe acute pancreatitis; Qingyi decoction; interleukin-8;interleukin-10 　　 　　重症急性胰腺炎（severe acute pancreatitis，SAP）是由多因素诱发、多个脏器受累、病情凶险、发展迅速、死亡率高的内科急重症，而多器官功能障碍综合征（multiple organ dysfun