生物素化微细纤维肽修饰IGF―1对心肌梗死兔肌球蛋白基因表达影响.docVIP

生物素化微细纤维肽修饰IGF―1对心肌梗死兔肌球蛋白基因表达影响 　　[摘要]目的 研究生物素化微细纤维肽修饰IGF-1对心肌梗死兔肌球蛋白基因表达的影响。方法 将28只健康的雄性新西兰白兔随机分为对照组、实验组1、实验组2、实验组3，每组7只。对照组开胸但不进行冠状动脉左前降支结扎，实验组1、2、3建立兔心肌梗死模型。建模成功后，实验组1注射生物素化微细纤维肽修饰的IGF-1，实验组2注射未修饰的IGF-1，对照组（未结扎）与实验组3（心肌梗死）注射同等剂量的生理盐水。术后8周，测定心肌肌球蛋白基因表达。结果 与对照组比较，实验组3心肌肌球蛋白基因的表达水平显著降低，实验组1、2心肌肌球蛋白基因的表达水平显著提高（P0.05），但实验组1的心肌肌球蛋白基因表达水平又显著高于实验组2（P0.05）。结论 急性心肌梗死会减弱兔心肌肌球蛋白的基因表达，IGF-1和生物素化微细纤维肽修饰IGF-1均能改善心肌梗死兔肌球蛋白的表达，但经过生物素化微细纤维肽修饰的IGF-1比单纯IGF-1的效果更优。 　　[关键词]生物素化微细纤维肽修饰IGF-1；心肌梗死；肌球蛋白；基因表达 　　[中图分类号] R332 [文献标识码] A [文章编号] 1674-4721（2017）10（a）-0033-03 　　[Abstract]Objective To study the influence of biotinylated microfibrinopeptide modified IGF-1 on myosin gene expression in rabbits with myocardial infarction.Methods A total of 28 healthy male New Zealand white rabbits were randomly divided into the control group，experimental group 1，experimental group 2，and experimental group 3，and there were 7 ones in each group.In the control group，rabbits were performed with thoracotomy but without ligation of left anterior descending branch of coronary artery.For experimental group 1，2，and 3，model of myocardial infarction in rabbits was established.After successful modeling，biotinylated microfibrinopeptide modified IGF-1 was injected in the experimental group 1.In the experimental group 2，biotinylated microfibrinopeptide IGF-1 without modification was injected.In the experimental group 3 （myocardial infarction） and control group （without ligation），the same dose of normal saline was injected.The gene expression of myosin was determined 8 weeks after surgery.Results Compared with the control group，theexpression level of myosingene in the experimental group 3 was significantly decreased，but in the experimental group 1 and 2，the gene expression level were greatly increased （P0.05）.The expression level of myosin gene in the experimental group 1 was significantly higher than that in the experimental group 2.Conclusion Acute myocardial infarction will attenuate the gene expression of m