白藜芦醇对C3H10T12间质干细胞成骨分化及CXCL12EGFR和CCL2基因表达影响.docVIP

白藜芦醇对C3H10T12间质干细胞成骨分化及CXCL12EGFR和CCL2基因表达影响 　　[摘要] 目的 探讨白藜芦醇对C3H10T1/2细胞成骨分化的作用及CXCL12、EGFR和CCL2基因表达的影响。 方法 采用CCK-8法检测不同浓度白藜芦醇对C3H10T1/2细胞增殖影响，碱性磷酸酶染色鉴定早期成骨分化，实时荧光定量PCR法检测CXCL12、EGFR和CCL2基因的表达。 结果 白藜芦醇在20 μmol/L浓度对C3H10T1/2细胞增殖没有影响（P 0.05），随着浓度增加，40、80、100 μmol/L的白藜芦醇明显抑制细胞增殖（P 0.05）。 结论 白藜芦醇能促进rhBMP-2诱导成骨分化。促成骨分化作用可能不是通过调控CXCL12、EGFR和CCL2基因的表达来实现。 　　[关键词] 早期成骨分化；间质干细胞；基因表达；白藜芦醇 　　[中图分类号] R285；R966 [文献标识码] A [文章编号] 1673-7210（2014）08（c）-0009-04 　　Effects of resveratrol on early osteoblast differentiation and CXCL12， EGFR and CCL2 gene expression of C3H10T1/2 mesenchymal stem cell 　　ZUO Changqing1 ZHONG Yuechun1 WANG Zonggui2 DAI Zhong1 WU Tie1 　　1.Department of Pharmocology， Guangdong Medical College， Guangdong Province， Dongguan 523808， China； 2.Department of Biochemistry， Guangdong Medical College， Guangdong Province， Dongguan 523808， China 　　[Abstract] Objective To study the effects of resveratrol on early osteoblast differentiation and mRNA expression of CXCL12， EGFR and CCL2 of C3H10T1/2 mesenchymal stem cells. Methods The C3H10T1/2 cell proliferation was detected using CCK-8 after different concentrations of resveratrol. The early osteoblast differentiation was identified by alkaline phosphatase （ALP） staining. The mRNA expression of CXCL12， EGFR and CCL2 was detected using real time quantitative RT-PCR. Results The resveratrol less than 20 μmol/L concentrations had no effect on C3H10T1/2 cell proliferation （P 0.05）， however， high concentrations of resveratrol （40， 80， 100 μmol/L） inhibited the C3H10T1/2 cell proliferation （P 0.05）. Conclusion Resveratrol can promote the rhBMP-2-induced osteogenic differentiation， but which may not act through regulating the mRNA expression of Cxcl12， Egfr and Ccl2. 　　[Key words] Early osteoblastic differentiation； Mesenchymal stem cells； Gene expression； Resveratrol 　　骨质疏松症是世界范围内的常见病、多发病。促进间质干细胞成骨定向分化与成熟是治疗骨质疏松症的新的有效手段。白藜芦醇是一种含有芪类结构非黄酮类多酚化合物，具有促进间质干细胞向成骨细胞方向分化，并抑制间质干细胞向脂肪细胞分化潜能[1]，但白藜芦醇促成骨分化机制尚未完全阐明。 　　间质干细胞成骨分