芪棱汤对局灶性脑缺血再灌注损伤大鼠5BrdU蛋白表达影响.docVIP

芪棱汤对局灶性脑缺血再灌注损伤大鼠5BrdU蛋白表达影响 　　【摘要】目的:探讨芪棱汤对局灶性脑缺血再灌注大鼠5-BrdU蛋白表达的影响。方法: 将大鼠随机分为假手术组、模型组、芪棱汤去养阴药组及芪棱汤组。采用颈内动脉线栓加环扎法复制大鼠局灶性脑缺血再灌注模型(MCAO\Reperfusion)。在再灌注后不同时间点采用免疫组化法检测5-BrdU蛋白表达。结果:芪棱汤去养阴药汤组与芪棱汤组5-BrdU蛋白表达明显多于模型组(P 0.05),且芪棱汤5-BrdU蛋白表达明显多于芪棱汤去养阴药汤组(P 0.05)。结论: 芪棱汤能够促进5-Brdu蛋白表达,其可能通过刺激内源性神经干细胞增殖达到神经保护作用。 　　【关键词】芪棱汤;局灶性缺血再灌注损伤;益气养阴活血法;5-嗅脱氧尿苷嘧啶 　　 　　Effects of Qileng Decoction on the Expression of 　　5-BrdU protein in Rats with Focal Cerebral Ischemia-Reperfusion Injury 　　ZHOU Rong?feng1 ZHAO Xi?lian1 DENG Qian1 JING Hong?yu2 　　【Abstract】Objective:To study the effect of the expression of 5-BrdU protein in Qileng decoction on brain after focal cerebral ischemia reperfusion rat. Method:The rats were randomly divided into 4 groups including thesham-operation group,the MCAO (Middle Cerebral Artery Occlusion)group, the group of Qileng Decoction without nourishing-Yin herbs and theQileng Decoction group.the MCAO model was established with method of internal carotid artery occlusion by suture. 5-BrdU were observed with immunohistochemistry method at different reperfusion time.Result: The expression of 5-BrdU protein in model group was significantly lower than that in both Qileng Decoction without nourishing-Yin herbs group and QiLeng decoction group (P0.05), and the expression of 5-BrdU in QiLeng decoction group were increased significantly compared with the group of Qileng Decoction without nourishing-Yin herbs(P0.05). Concolusion:QiLeng decoction play the neuron protective effects by increasing the expression of the 5-BrdU protein. 　　【Key words】Qileng decoction;Focal cerebral ischemia-reperfusion injury; Benefiting- Qi for Activating Blood Circulation,and Nourishing- Yin;5-BrdU 　　【中图分类号】R743 【文献标识码】A 【文章编号】1006-1959(2009)08-0092-02 　　 　　局灶性脑缺血发生后,梗死核心区的神经元很快死亡,周边组织相继出现神经元凋亡,临床上出现相应的神经功能缺损表现。寻找有效促进神经功能恢复的药物长期以来一直是临床和实验研究的热点。以往认为,成熟神经系统的神经元是终极细胞,受损后不能再生,而目前越来越多的研究表明,成年人脑内存在具有自我更新和多种分化潜能的神经干细胞,从而为卒中的治疗带来新希望。本课题组前期研究证明了芪棱汤可以促进神经干细胞的特异性标志物nestin蛋白的表达[1]。本研究旨在观察芪棱汤对