淀粉残渣木质纤维素降解机制与产品开发研究-微生物学专业论文.docxVIP

华中科技大学硕士学位论文 华 中 科 技 大 学 硕 士 学 位 论 文 II II Abstract The residue of radix puerariae, wheat bran and residue of pachyrhizus were the byproducts in the process of producing starch. Starchy content of them were different and they could be used as material to produce dietary fibre. This paper studied on the changes of the enzyme activity, content of the lignocelluose and the dietary fibre during the fermentation process by fungi. On the residue of radix puerariae medium, the strain DS degraded cellulose priority and the degraded quality of the cellulose could reach 5.527g during 12 days incubation, then hemicellulose could be degraded by 0.437g on the first 6 days, 0.034g of lignin was degraded by the white rot fungus, furthermore, laccase activity had a low level and the activity was 164U/mL on the third day and decreased gradually with the increasing of incubation time. On the medium containing wheat bran, the hemicellulose was degraded firstly by strain DS and the value of degradation was 2.303g, the biodegradation mass of the cellulose increased, but the lignin had not been degraded. Laccase activity secreted by strain DS had a high level, and the activity could up to 16030U/mL on the 6th day. On the medium containing residue of pachyrhizus, the strain DS could degrade some lignocelluose. The degradation quality of cellulose could increase by 1.506g during the first 15 days incubation, the value of the hemicellulose was increase, and the lignin had been degraded by 0.395g. In addition, laccase activity had a low level and the activity was 14U/mL on the 6th day. Based on the foundation of fermentation law by fungi in different media, the sanitarian medicine with the residue of radix puerariae was investigated. The fermentation effect would be better under appropriate conditions, such as water addition was 150% and the substrate was downy. Under these conditions, the content of flavone, soluble dietary fibre and insoluble dietary fibre could reach 37.140mg/g , 13.2%and