醋甘遂活性部位各成分群对斑马鱼胚胎急性毒性比较研究.docVIP

醋甘遂活性部位各成分群对斑马鱼胚胎急性毒性比较研究 　　[摘要]采用模式生物斑马鱼的胚胎评价醋甘遂活性部位各成分群急性毒性，同时采用UFLCQTOFMS对各成分群的化学成分进行定性研究。以24 h健康斑马鱼胚胎为研究对象，各成分群设9个浓度和1个空白对照组，考察给药后96 h其对斑马鱼胚胎存活率的影响，计算不同样品对斑马鱼胚胎的半数致死浓度（LC50）。结果显示，各成分群对斑马鱼胚胎的急性毒性大小依次为BCAD，成分群D的毒性不明显。UFLCQTOFMS分析结果显示成分群B主要检测到巨大戟烷型二萜类成分，成分群C主要检测到假白榄烷型二萜类成分。关联毒性研究结果，推测假白榄烷型二萜类成分可能为醋甘遂中更为低毒的活性成分，可为进一步深入探究醋甘遂“有故无殒”的物质基础提供依据。 　　[关键词]醋甘遂； 成分群； UFLCQTOFMS； 斑马鱼胚胎； 急性毒性 　　Comparative study on acute toxicity of different active ingredient 　　fractions from Kansui stirbaked with vinegar on zebrafish embryos 　　LOU Jianwei， CAO Liangliang， ZHANG Qiao， ZHANG Li*， DING Anwei 　　（Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization， National and 　　Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine， 　　Nanjing University of Chinese Medicine， Nanjing 210023， China） 　　[Abstract]The 24 h normal developing zebrafish embryos were used to evaluate the acute toxicity and the compounds of respective fractions were analyzed by UFLCQTOFMS simultaneously Nine concentration groups with respective concentration and a blank control group were designed for each fraction to investigate their effect on survival rates of zebrafish embryos 96 h after drug administration， and calculate the median lethal concentration （LC50） of different fractions to zebrafish embryos The results showed that all of the fractions had acute toxicity to zebrafish embryos except VEKD， and the order was as follows： VEKB， VEKC， VEKA and VEKD According to the results of UFLCQTOFMS， the chemical ingredients contained in VEKB and VEKC were mainly composed of ingenanetype and japhanetype diterpenoids， respectively It could be speculated that japhanetype diterpenoids might be the active compounds with lower toxicity associated with the results of toxicity study， providing some references for the further research on effective material basis of Kansui stirbaked with vinegar according to the principle of