好氧堆肥中高温纤维素分解菌的筛选及性状研究中国科技论文在线.PDFVIP

中国科技论文在线 ( ) 北京大学学报 自然科学版 ,第 42 卷 ,第 2 期 ,2006 年 3 月 Acta Scientiarum Naturalium Universitatis Pekinensis , Vol . 42 , No . 2 (Mar . 2006) 1) 好氧堆肥中高温纤维素分解菌的筛选及性状研究 王志超 　陆文静 　王洪涛 (清华大学环境科学与工程系 , 北京 , 100084) 摘 　要 　从规模化好氧堆肥的高温阶段筛选获得 6 株具有较高纤维素降解能力的高温菌 。这些菌株的生长速率 、 温度稳定性以及纤维素酶活性检测结果表明 ,它们能在 45～65 ℃的温度下生长 ,最适生长温度为 50～60 ℃;在普 ( ) 通细菌培养基和以羧甲基纤维素钠 CMC 为唯一碳源的限制性培养基上均能够快速启动并旺盛生长 ,分别在培养 14 h 和 36 h 左右达到最大活菌数 。纤维素酶活性检测结果表明 ,获得的菌株均具有较高的 C 酶活和滤纸崩解能 力 ,能够在 2～3 d 内快速启动滤纸条崩解 ,并显著降低滤纸条中纤维素含量 。 关键词 　高温 ; 纤维素分解菌 ; 筛选 ; 纤维素酶 中图分类号 　X 172 Research on the Isolation and Characters of Several Strains of Thermophilic Cellulolytic Bacteria WAN G Zhichao 　L U Wenj ing 　WAN G Hongtao ( Dep artment of Environmental Science and Engineering , Tsinghua University , B eij ing , 100084) ( ) Abstract 　Six strains of thermophilic cellulolytic bacteria TCB which can strongly decompose celluloses were isolated in the high temperature period from a largescale composting. Their rate of growth , stability of temperature and activity of cellulase were determined. The results showed that they could grow under the temperature from 45 ℃to 65 ℃, and the optimum temperature is 50 ℃60 ℃. They could grow rapidly and vigorously on both the general culture medium for bacteria and the restrictive medium in which CMC is the