银杏叶提取物对睡眠剥夺小鼠学习记忆能力影响及其机制研究.docVIP

银杏叶提取物对睡眠剥夺小鼠学习记忆能力影响及其机制研究 　　[摘要] 目的 研究银杏叶提取物（EGB）对睡眠剥夺小鼠学习记忆能力的影响及其机制。 方法 选取成年健康清洁级小鼠48只，雌雄各半，根据随机数字表法将小鼠随机分为4组（n=12）：空白组，对照组，治疗Ⅰ组，治疗Ⅱ组，适应性饲养3天，每组筛选出9只进行造模。应用72 h睡眠剥夺法制作动物模型，用Y迷宫成绩（正确反应次数）评定学习记忆能力，免疫组织化学法反映小鼠海马脑源性神经营养因子（BDNF）的蛋白表达。 结果 Y迷宫测试结果显示，给药后，对照组与治疗Ⅰ组、对照组与治疗Ⅱ组的正确反应次数差异有统计学意义（P0.05）。与对照组比较，空白组、治疗Ⅰ组、治疗Ⅱ组海马区的BDNF表达增高，其灰度值差异有统计学意义（P0.05）。 结论 睡眠剥夺能造成脑损伤、学习记忆能力减退以及BDNF表达减少，EGB可能是通过上调BDNF蛋白及其相关通路蛋白改善睡眠剥小鼠的学习记忆能力。 　　[关键词] 银杏叶提取物；睡眠剥夺；脑源性神经营养因子；学习记忆 　　[中图分类号] R332 [文献标识码] A [文章编号] 1674-4721（2016）02（c）-0004-04 　　[Abstract] Objective To study the influence extract of Ginkgo biloba （EGB） on learning and memory ability in sleep-deprivation mice and its mechanism. Methods Forty-eight healthy and clean adult mice were selected，half male and half female，and they were evenly divided into 4 groups according to the random number table method：blank group，control group，treatment groupⅠ， and treatment group Ⅱ，and they were given adaptive feeding for 3 days，9 mice were selected in each group to make model.72-hour of sleep-deprivation method model was applied.The learning and memory ability was evaluated by achievement of Y maze.The protein expression of brain-derived neurotrophic factor （BDNF） in hippocampal area in mice was reflected by immunohistochemistry. Results Test result of Y maze showed that，after administration，there was a statistical difference of right reaction times between control group and treatment groupⅠ，and between control group and treatment groupⅡ （P0.05）.Compared with control group，BDNF expression of hippocampal area in blank group，treatment group Ⅰ，and treatment group Ⅱ was improved，and there was a statistical difference of gray value （P0.05）. Conclusion Sleep-derivation can lead to cerebral damage，decreased learning and memory ability，and reduction of BDNF expression，and EGB may improve learning and memory in sleep-deprivation mice by up-regulating BDNF protein and its related pathway protein. 　　[Key words] Extract of Ginkgo biloba；Sleep-deprivation；Br