茯砖茶有机酸反相高效液相色谱分析方法建立及其应用.PDFVIP

中国农学通报 2011,27(30):246-252 Chinese Agricultural Science Bulletin 茯砖茶有机酸反相高效液相色谱分析方法建立及其应用 袁 玲，黄建安，龚志华，陈金华，李银花，袁 勇，肖文军 （湖南农业大学茶学教育部重点实验室/ 国家植物功能成分利用工程技术研究中心，长沙410128 ） 摘 要：为建立一种简便快速的高效液相检测法测定茯砖茶有机酸，采用Kromasil C 18 色谱柱（5 μm ， 250 mm×4.6 mm ），色谱条件为：3% CH OH- 97% 0.1 mol/L KH PO （用磷酸调pH 至2.7 ）作为流动相，流 3 2 4 速0.4 mL/min ，柱温17℃，进样量10 μL ，检测波长210 nm 。该方法回收率达到90.12%~105.74% ，相对标 准偏差小于1.80% ，各有机酸浓度与HPLC 峰面积的线性相关系数大于0.998 ，表明所用方法快速简便， 具有较高的准确度和精密度。应用此方法测定茯砖茶样品中有机酸组成与含量，结果11种有机酸在茯 砖茶中均能检出。 关键词：反相高效液相色谱；茯砖茶；有机酸 中图分类号：TS272 ，O65.7+2 文献标志码：A 论文编号：2011-1960 Study on Development and Application Technology of Analysis Method for Organic Acid in Fu-brick Tea by RP-HPLC Yuan Ling, Huang Jian ’an, Gong Zhihua, Chen Jinhua, Li Yinhua, Yuan Yong, Xiao Wenjun (Key Lab of Tea Science of Ministry of Education, Hunan Agricultural University/National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanical, Changsha 410128) Abstract: To establish a kind of simple and rapid high performance liquid chromatography determination method of organic acid in fu-brick tea, Kromasil C18 column was used at 17℃ and mobile phase was 3% CH OH -0.1 mol/L KH PO (pH value adjusted with phosphoric acid to 2.7) with flow rate of 0.4 mL/min. 3 2 4 10 μL sample was injected into the column and detection wavelength was 210 nm. The relative standard deviations were lower than 1.80%, and recovery was 90.12% to 105.74%. All of the correlation coefficients between the concentration of the organic acids and the HPLC r