骨桥蛋白在侵入性胎盘组织中表达和意义.docVIP

骨桥蛋白在侵入性胎盘组织中表达和意义 　　[摘要] 目的 分析骨桥蛋白（OPN）在侵入性胎盘和正常胎盘组织中的表达差异，探讨其在侵入性胎盘发病机制中的作用。 方法 选取2014年1月～2015年10月在中国人民解放军空军总医院妇产科住院分娩的单胎晚期妊娠68例产妇的胎盘组织为研究对象，将其中正常妊娠产妇的胎盘组织34例作为对照组，侵入性胎盘疾病产妇的胎盘组织34例作为研究组，其中胎盘粘连26例（胎盘粘连组），胎盘植入8例（胎盘植入组）。采用免疫组化EnVision二步法检测所有胎盘组织中OPN蛋白表达水平；采用SYBR Green PCR方法检测所有标本中OPN mRNA的表达水平。 结果 OPN蛋白在胎盘粘连组、胎盘植入组和对照组胎盘组织中的阳性表达率分别为73.07%、75.00%、35.29%。胎盘粘连组和胎盘植入组OPN蛋白阳性表达率均高于对照组，差异均有统计学意义（P 0.05）。胎盘粘连组中OPN mRNA表达较对照组上调1.36倍，而胎盘植入组OPN mRNA表达较对照组上调了1.21倍。 结论 胎盘组织中OPN表达升高可能与侵入性胎盘疾病的发生有关。 　　[关键词] 骨桥蛋白；侵入性胎盘；粘连性胎盘；植入性胎盘 　　[中图分类号] R339.22 [文献标识码] A [文章编号] 1673-7210（2016）03（b）-0080-04 　　[Abstract] Objective To investigate the role in thepathogenesis of placenta accrete through analyzing the expression differences of osteoponin （OPN） in the tissues of placenta accrete and normalplacenta. Methods Sixty-eight cases of placenta tissues of the third trimester pregnant women delivering in Department of Gynecology and Obstetrics of Air Force General Hospital from January 2014 to October 2015 were chosen as the research objects. 34 cases of placenta tissues from normal pregnancy women were taken as the control group and 34 cases of placenta tissues from pregnant women with placenta accrete were taken as the research group， of which including 26 cases of placenta adhesion （placenta adhesion group） and 8 cases of placenta increta （placenta increta group）. The protein expressions of OPN in all placenta tissues were measured by EnVision immunohistochemistry. The mRNA expression of OPN in all placenta tissues were detected through SYBR Green PCR method. Results The positive rates of OPN protein expression in the placenta adhesion group， placenta increta group and control group were 73.07%， 75.00%， 35.29% respectively. The positive rates of OPN protein expression in the placenta adhesion group and placenta increta group were both significantly higher than the control group， the differences were statistically significant （P 0.05）. The mRNA expression of OPN in placenta adhesion g