骨化三醇对脂肪变性肝细胞三酰甘油代谢及细胞因子信号转导抑制因子―3基因表达影响.docVIP

骨化三醇对脂肪变性肝细胞三酰甘油代谢及细胞因子信号转导抑制因子―3基因表达影响 　　[摘要] 目的 研究骨化三醇对脂肪变肝细胞三酰甘油代谢及细胞因子信号转导抑制因子-3（SOCS-3）基因表达的影响。 方法 以油酸（OA）诱导正常人肝细胞LO2脂肪变为模型，按加入骨化三醇浓度的不同（10-8、10-7、10-6 mol/L）分为三个干预组，设空白对照组。干预组先加入不同浓度骨化三醇干预24 h，之后加0.2 mmol/L的OA 24 h。检测细胞内三酰甘油（TG）、上清液谷草转氨酶（AST）含量，采用实时荧光定量PCR检测各组细胞SOCS-3 mRNA的表达，多组间比较采用单因素方差分析进行数据分析。 结果 模型组TG[（362.43±22.20）mg/dL]、AST[（5.55±0.46）U/L]、SOCS-3 mRNA表达（4.61±0.39）均较对照组[（175.78±7.69）mg/dL、（0.63±0.33）U/L、（1.00±0.00）]明显增高（P 0.01）；干预组TG分别为（286.87±16.01）、（257.16±11.73）、（209.43±27.79）mg/dL，AST分别为（4.98±0.50）、（4.23±0.36）、（3.50±0.48）U/L，SOCS-3 mRNA表达分别为（3.71±0.69）、（2.67±0.70）、（1.31±0.42），较模型组均有所下降，差异均有统计学意义（均P 0.05）。 结论 骨化三醇可抑制肝细胞脂肪变，其作用机制可能与骨化三醇抑制SOCS-3的表达有关。 　　[关键词] 骨化三醇；LO2细胞；三酰甘油；细胞因子信号转导抑制因子-3 　　[中图分类号] R575.2 [文献标识码] A [文章编号] 1673-7210（2014）04（a）-0064-03 　　Effects of Calcitriol on triglyceride metabolism and SOCS-3 gene expression in fatty degeneration of liver cells 　　HUANG Han1 ZHAO Longfeng2 LI Hong2 　　1.Graduate School， Shanxi Medical University， Shanxi Province， Taiyuan 030000， China； 2.Department of Infectious Diseases， First Hospital of Shanxi Medical University， Shanxi Province， Taiyuan 030000， China 　　[Abstract] Objective To investigate the effect of Calcitriol on triglyceride metabolism and suppressor of cytokine signalling-3 （SOCS-3） gene expression in fatty degeneration of liver cells. Methods A steatosis atocytes model was established in LO2 cells induced with oleic acid （OA）. The cells were divided into three intervention groups by added different concentrations of Calcitriol （10-8， 10-7， 10-6 mol/L）， cells without any treatment were considered as normal control group. The cells were treated with different concentrations of Calcitriol for 24 h， after which they were cultured with OA （0.2 mmol/L） for another 24 h. The intracellular triglyceride （TG） and AST levels were observed as indicators of hepatic steatosis. The expression of SOCS-3 mRNA of each group were measured by real-time PCR. When more than two groups were compared， one-w