紫穗槐果实里天然成分的提取论文--毕业论文设计.docVIP

西南科技大学本科生毕业论文 西南科技大学本科生毕业论文 Ⅰ 紫穗槐Amorfrutin的提取与分析 摘要：Amorfrutin是一种具有抗癌活性的苯基类化合物，且具有一定的抗糖尿病效应。这种天然活性物质不仅具有降血糖、抗炎症的功效，还具有很好的耐受性。 Amorfrutin及其类似物还可以预防脂肪肝-一种因过量高脂营养引起的常见病，而且可被用作功能性的营养补剂或作为为患者量身定制的温和性治疗药物成分。Amorfrutin最早发现于原产于北美的甘草及紫穗槐果实里，而对于国内紫穗槐是否也含有这种天然产物，则需要进一步研究。本实验对国内引进栽培的紫穗槐的果实里是否含有Amorfrutin这种物质进行了探究。根据文献调研，采取溶剂萃取法，用无水乙醇对紫穗槐果实中的化学物质进行提取后，通过液相质谱联用法对萃取液成分进行分析鉴定，得到提取物的质谱图，证实了提取物中确实含有Amorfrutin。然后改变实验条件及提取工艺，先将紫穗槐果实用5%的盐酸和氯仿进行萃取，再经石油醚和甲醇萃取，经液相质谱联用分析，在甲醇相中得到了Amorfrutin的谱图。最后再将两次不同结果图谱的质荷比和相对丰度进行对比，证实对比实验中经萃取处理后的紫穗槐果实中提取到更多的Amorfrutin，初步找到提取紫穗槐果实里Amorfrutin最优工艺。 关键词：天然产物；紫穗槐；萃取；Amorfrutin；糖尿病 西南科技大学本科生毕业论文 Ⅱ The extraction and analyses of Amofrutin from Amorpha fruticosa Abstract: Amorfrutin as an anti-cancer activity of phenyl compound, has a certain anti-diabetic effects. According the world latest discovery, this natural active substance with hypoglycemic, anti-inflammatory effect, also has good resistance effect at the same time. Amorfrutin and its analogues can also prevent fatty liver, a common disease caused by the excessive of fat nutrition. And it can also be used as functional nutritional supplements or a mild treatment to the patients. The natural substance Amorfrutin was first discovered in the plant of licorice, and then in the seed of Amopha fruticosa with its effect of ant-diabetic. But it still need further study to make sure whether the domestic Amorpha has the Amorfrutin or not. In this paper, we studied this issue. We use the solvent extraction in the anhydrous ethanol to extract Amorfrutin from the seed of Amorpha, and then washed with hydrochloric (5%) acid and methanol. By using liquid phase chromatography to appraise the Amorpha, we find it contains Amorfrutin in the solution after ethanol extraction. After comparing di