应用浊度测定法於土壤微生物Pseudomonasfluorescens对饱和水力.PDF

應用濁度測定法於土壤微生物 Pseudomonas fluorescens 對飽和水力傳導度影響之研究 (1) (2) (2) 林俐玲 杜怡德 蔡義誌 摘 要 本研究目的在瞭解吸光法應用於量測微生物 Pseudomonas fluorescens 生長所產生孔隙 阻塞，造成水力傳導度降低之可行性。研究中以石英砂來模擬土體在飽和狀況下飽和水力 傳導度（Ks 值）之變化，為此進行微生物影響飽和水力傳導度實驗及純水對照實驗，並以 抬升水頭及加入殺菌劑等不同處理觀察對飽和水力傳導度的影響。結果顯示吸光法可適用 於微生物對飽和水力傳導度影響之研究，不失為一簡單可行又具相當準確度之方法。 關鍵字( ：Pseudomonas fluorescens 、飽和水力傳導度 、生物性阻塞) Assessing the Effect of Pseudomonas Fluorescens on Saturated Hydraulic Conductivity with Turbidity Method Li-Ling Lin(1), Yi-Te Tu(2), Yi-Zhih Tsai(2) Professor and Chairperson , Ph.D Graduated Students , Department of Soil and Water Conservation,National Chung Hsing University , Taichung, Taiwan 402, R.O.C. ABSTRACT This study is focused on the usability of using Turbidity Method in assessing the effect of microorganisms clog pores (Bioclogging) on soil saturated hydraulic conductivity (Ks value) with the sand column. Adding Pseudomonas fluorescens or water (controlled experiment) into the sand column with full nutrition were conducted with three conditions : elevated hydraulic head 3 cm, elevated hydraulic head 2 cm and add sodium azide ，NaNO3,a strong biocide. (1)國立中興大學水土保持學系教授兼系主任 (2)國立中興大學水土保持學系博士研究生 123 水土保持學報 39(2) ：123-131 (2007) Journal of Soil and Water Conservation , 39(2) ：123-131 (2007) The results showed that, Pseudomonas fluorescens caused decrease in the saturated hydraulic conductivity. It suggests that the Turbidity Method may be a good method to assess the growth of Pseudomonas fluorescens as related to the decreased saturated hydraulic conductivity . (Key words: Pseudomonas fluorescens, Turbidity Method, Saturated hydraulic conductivity )