二甲戊乐灵降解细菌HB27的分离及降解特性研究.PDFVIP

第 24 卷第 2 期 环 　境 　科 　学 　学 　报 Vol . 24 ,No . 2 2004 年 3 月 ACTA SCIENTIAE CIRCUMSTANTIAE Mar . ,2004 ( ) 文章编号 2004 　　　中图分类号 :X53 　　　文献标识码 :A 二甲戊乐灵降解细菌 HB7 的分离及降解特性 研究 ( 山东农业大学环境科学系 ,泰安 　271018) 朱鲁生 ,林爱军 ,王 　军 ,孙瑞莲 ,刘爱菊　 ( ) 摘要 :利用富集培养方法 ,从环境中获得菌株 HB7 ,初步鉴定属于芽孢杆菌属 B acillus . 实验结果表明,培养条件会影响 HB 7 的生长和降解二甲戊乐灵的效率 ,少量的蔗糖能促进菌体生长和降解 ;在p H 为 6～9 的范围内菌体生长量大 、降解率高 ;二 甲戊乐灵浓度的提高抑制了菌株的生长和降解 ,但在一定浓度范围内提高农药去除量 ;HB7 降解二甲戊乐灵的最佳培养温 度是 30 ℃左右. 研究结果还表明菌株 HB7 含有一个质粒 ,该质粒能产生降解二甲戊乐灵的酶系统. 关键词 :二甲戊乐灵 ; 芽孢杆菌 ; 降解 ; 生长量 Isolation of pendimethalin degrading bacteria HB7 and its research of degrading characteristics ( ) ZHU Lusheng , LIN Aij un , WANGJ un , SUN Ruilian , LIU Aij ü　 Shandong Agricultural University , Taian 　271018 Abstract :Microbial degradation of pendimethalin and the effects of culture conditions in vitro were studied. Bacteria HB7 considered as an ef ficient degrading strain was isolated by enrichment from environment identified as B acillus . The efficiency of degradation was affected by cul ture conditions such as p H ,temperature and the concentration of sucrose and pendimethanlin . The optimal conditions were proposed. The re sults showed that when additional carbon source at 01 % ,p H value at 6～9 and cultivated temperature at 30 ℃, HB7 growth and degradation rate were enhanced. HB7 growth was inhibited but the removal was enhanced by pendimethalin . By using agarose gel electrophoresis tech nique strain HB7 was found to harbour one plasmid about 20 K which c