杜氏盐藻生理生化鉴定及高质量cDNA文库表达序列标签分析-生物医学工程专业论文.docxVIP

大量未鉴定的新基因。大约 21%的 ESTs（功能注释 ESTs 的 44%）编码与蛋白质合 成和加工功能有关的蛋白（主要是蛋白质合成装置的成分核糖体蛋白），表明核糖 体蛋白在盐藻细胞生命过程和耐盐调节中的重要作用。ESTs 分析中除发现了大量与 杜氏盐藻的蛋白质合成和加工功能有关的 ESTs 外，还发现了许多编码能量代谢与光 合作用（功能注释 ESTs 的 21.2%）以及初级代谢（功能注释 ESTs 的 5.5%）功能相 关的 ESTs。此外，也发现了一些与细胞转运，细胞信号和细胞周期，细胞结构组分 以及细胞抗逆性等功能有关的 ESTs。同时将该研究中得到的 ESTs 与莱茵衣藻，团 藻进行功能基因组学对比，发现其中 245 个 ESTs 与两者的已知功能序列具有同源性， 提示其余 ESTs 很可能代表耐盐相关的特异性新基因。最后对这些 ESTs 所进行的简 单全长分析还得到几个未在杜氏盐藻中报道过基因的全长编码序列。 关键词：杜氏盐藻，培养条件，生长曲线，高质量 cDNA 文库，表达序列标签（ESTs）， 耐盐性 Abstract The unicellular, halotolerant, green alga, Dunaliella salina (Dunaliellaceae, Dunaliella) has no cell wall, but has the unique ability to adapt and grow in a wide range of salt conditions from about 0.05 to 5.5 M. Dunaliella salina is rich in various valuable bioactive substances, such as β-carotene, while can also produce large amounts of bio-oil under salt-stress conditions. Dunaliella salina is the best model organism to study molecular mechanisms of salt-tolerance since that it is the most salt-tolerant photoautotrophic eukaryote and also has simple cell structure. Currently, there are few research reports on indoor training system, microscopic morphology and cell growth curve of various Dunaliella species, which makes Dunaliella’s large-scale biotechnology application and basic molecular research relatively difficult. In the present study, we collected six Dunaliella species: Dunaliella salina, Dunaliella parva, Dunaliella primolecta, Dunaliella bardawil, Dunaliella tertiolecta and Dunaliella sp., established and improved culture conditions of the above six Dunaliella species to achieve the success of indoor cultivation. Based on the successful cultivation, microscopic morphology structure of six Dunaliella species was observed by inverted microscope, showing that all of six species are unicellular green algae, have round, oval or spindle shape, contain a large cup-like chloroplast, give birth to two long flagellum on the top, as well as that different species have different cell size and Duna