南瓜皮类胡萝卜素的提取工艺研究-中国农学通报.PDF

中国农学通报 2012,28(36):295-299 Chinese Agricultural Science Bulletin 南瓜皮类胡萝卜素的提取工艺研究 付 莉，王歆姬 （辽宁医学院，辽宁锦州121001） 摘 要：南瓜皮中富含功能性色素——类胡萝卜素，类胡萝卜素有抗氧化、防癌症、预防夜盲症、着色力强 等功能。为了提供类胡萝卜素的应用和稳定性研究基础，以南瓜皮为原料，对其中的类胡萝卜素进行提 取研究，以丙酮和石油醚为溶剂，采用超声波辅助溶剂提取，以提取温度、提取时间、提取料液比为三因 素进行三因素三水平正交实验，研究南瓜皮类胡萝卜素最佳提取工艺。结果表明，当超声波功率为 150 W 时，利用丙酮+石油醚为提取溶剂，在提取温度为30℃，提取时间为30 min ，提取料液比为1:10 时， 南瓜皮类胡萝卜素提取率最高。由此得出，利用混合溶剂辅助超声波提取南瓜皮类胡萝卜素，提取周期 短，提取率高，提取出的高纯度类胡萝卜素可以为后期的稳定性研究提供理论基础。 关键词：南瓜皮；类胡萝卜素；超声波；提取 中图分类号：TS2 文献标志码：A 论文编号：2012-1335 Extraction of Carotenoids from Pumpkin Peel Fu Li, Wang Xinji (Liaoning Medical College, Jinzhou Liaoning 121001) Abstract: The pumpkin peel contains abundent functional pigments-carotenoids, which have antioxidation, preventing cancer, preventing nyctalopia, strong color and many other functions. In order to provide theoretical foundation for the function and stability of carotenoids, We focused on the research of the extraction of carotenoids used pumpkin peel as raw material. We used acetone and petroleum ether as solvent assisted ultrasonic extraction, we used three factors and three levels orthogonal experiment to research on the optimum extraction technology of pumpkin peel carotenoids. Results showed that, when the ultrasonic power was 150 W, the best solwent extraction was acetone and petroleum, the largest absorption wavelength was 450 nm, the best extraction temperature was 30 centigrade, the optimum extraction time was 30 min, the best ratio of solid to liquid extraction was 1:10, the extraction rate of pumpkin peel was highest. Using mixed solven