原生质体融合构建耐高温高产酒精酵母.PDF

※生物工程 食品科学 2007, Vol. 28, No. 04 231 1 2 2 2 1 1 路玲玲 ，王 敏 ，朱会霞 ，孙金旭 ，檀建新 ，张 伟 (1.河北农业大学食品科技学院生物工程系，河北 保定 071001； 2.衡水学院生命科学系，河北 衡水 053000) ：选择化学药物和紫外灭活单亲原生质体为遗传标记，以0.01%的三唑酮和紫外照射h-1原生质体2min为 遗传标记，采用四因素三水平正交试验确定了融合子LZ-3的浓醪酒精发酵工艺。浓醪发酵的最佳工艺条件是：料 水比为1:2，糖化酶用量为 400U/g 原料，接种量为 9%(V/V)，初始pH值为5.5，在此条件下，以玉米粉的糖化醪 为培养基，发酵72h，LZ-3 的酒精产率为9.0%。 ：原生质体；融合；耐高温；高产酒精酵母 Building Thermotolerant High-alcohol-producing Yeast by Proplast Fusion 1 2 2 2 1 1 LU Ling-ling ，WANG Min ，ZHU Hui-xia ，SUN Jin-xu ，TAN Jian-xin ，ZHANG Wei (1.Department of Biology Engineering, College of Food Science and Technology, Agricultural University of HeBei, Baoding 071001, China；2.Department of Life Science, Hengshui University, Hengshui 053000, China) Abstract ：As chemical drugs and UV used to exterminate the single parent protoplasts were choosen as hereditary markers, with 0.01% triadimefon content and irradiated by UV 2 min, the optimum conditions of the orthogonal test are: 33.3%(W/V) concentration of crude corn in fermenting material, 400 U/g of glucoamylase (per gram of raw corn), 9%(V/V) inoculum of yeast, and initial pH 5.5. The orthogonal test indicated that the fusion strain LZ-3 produces the highest amount of alcohol, or 9.0% alcohol after incubation at 42℃ for 72 hours. Key words：protoplast；fusion；thermotolerant；high-alcohol-producing yeast 中图分类号：TS201.3 文献标识码：A 文章编号：1002-6630(2007)04-0231-06