植酸酶基因工程研究进展-生命科学研究.PDF

7 2 () 生命科学研究 Vol.7 No. 2(Suppl. ) 2003 6 Life Science Research June 2003 ¹ 刘生峰, 彭远义 ( , 400716) : 植酸酶是催化植酸及植酸盐水解成肌醇和无机磷酸的一类酶的总称. 添加于 品和饲料中, 能消除 植酸所引起的抗营养作用, 可提高蛋白质和矿物质的生物利用率. 对植酸酶的生物学特性基因结构和基因工 程的研究进展做了综述. : 植酸; 植酸酶;基因工程 : Q556 : A : 1007-7847(2003) S0-0030-04 Advances in Gene Engineering of Phytase LIU Sheng-feng, PENG uan-yi ( College of Animal Science Technology, SouthwestAgricultural University, Chongqing 400716, China) Abstract: Phytases are enzymes capableof hydrolyzing phytic acid (or phytate)to less-phosphorylated myo-inositol derivates and inorganic phosphate. The addition of phytase to feed and food can resolve the antinutrition of phytic acid and increase the bioavailablity of protein and minerals. The progresses in the recent studies related to the biologic characterizations, the structure of its gene , and the gene engineering of phytase was reviewed. Key words: phytic acid; phytase; gene engineering (Lfie Science Research,2003,7(2) :30~ 33) ., ,, 50% ~ 70% . ( ) , 1 , . , , , 2+ 2+ 2+ Mg ,Zn ,Ca 3 :3-( EC3. 1.3. 8) 6-(EC3. , , 1.3. 26) [ 1] [2] . (EC3. 1.3.2) . ,, ,, Km , pH [3~ 6] ,, . , , , ,30~ 250 kD ,