不同产区和采收期黄柏中小檗碱和黄柏碱含量对比研究.docVIP

不同产区和采收期黄柏中小檗碱和黄柏碱含量对比研究 　　[摘要] 目的 对不同产区与采收期黄柏药材中小檗碱与黄柏碱含量进行对比研究，为其药材质量评价与资源合理利用提供参考。 方法 采用高效液相色谱法对不同产区与采收期黄柏中小檗碱与黄柏碱进行测定。[关键词] 黄柏；产地；采收期；小檗碱；黄柏碱 　　[中图分类号] R284 [文献标识码] A [文章编号] 1673-7210（2014）04（b）-0090-04 　　[Abstract] Objective To make a contrastive study about comparing the contents of berberine and phellodendrine in Cortex phellodendri of different product districts and picking time， and provide information for its medical material quality appraise and the reasonable utilization of resources. Methods HPLC was used to determine the contents of berberine and phellodendrine in Cortex phellodendri of different product districts and picking time， the chromatographic column was Lichrospher detect wavelength was 284 nm， the flow rate was 1 mL/min. Results In the detected 22 batch samples， while there was a certain difference in Cortex phellodendri of different product districts， all got product districts， and could reach the limit requirements of the Cortex phellodendri content in Chinese Pharmacopeia （2010 edition）. The maximum content of Cortex phellodendri were in Baojing and Huatan County； picking time had certain effect， the sample gathered in May and the first ten days of June had higher Cortex phellodendri contend， and the simple gathered in the last ten days of June and the middle ten days of August had much lower Cortex phellodendri content. Conclusion Cortex phellodendri content in Baojing and Huatan county is higher， and the best adduct time is May and the first ten days of June. 　　[Key words] Cortex phellodendri； Product district； Picking time； Berberine； Phellodendrine 　　黄柏为芸香科植物黄皮树Phellodendron chinense Schneid.的干燥树皮，具有清热燥湿、泻火除蒸、解毒疗疮的功效，是临床常用的清热燥湿药，主治湿热泻痢、黄疸、热淋、湿疹症。黄柏习称“川黄柏”，分布于湖北、湖南西北部、云南、四川东部等地[1]，小檗碱、黄柏碱等是其主要药效成分，具有抗菌、消炎等药理作用[2-3]。目前，中药材市场存在问题较多，黄柏同种药材或饮片质量参次不齐，区域差异、种植的规范化及不按规律采收与加工炮制等[4]均在一定程度上影响药材的有效成分含量。试验收集了四川、云南、贵州、湖南等地不同时间采收的22批次黄柏药材，采用高效液相色谱（HPLC）法对其小檗碱、黄柏碱含量进行了检测[5]，以期为黄柏药材质量评价与资源合理利用提供试验数据与参考。 　　3 讨论 　　3.1 样品采集 　　黄皮树有雌株与雄株之分，两者含量有一定的区