肺炎链球菌体内诱导基因spd1672影响磷壁酸合成和细菌生长1黄健1.DOCVIP

肺炎链球菌体内诱导基因spd1672影响磷壁酸合成和细菌生长 [基金项目] 国家自然科学基金（ [基金项目] 国家自然科学基金（No [通信作者]王虹，Email:wanghongljf@163.com 黄健1，张彦青1，黄美容2，吴凯锋1，胥文春1，尹一兵1，王虹1# 1重庆医科大学检验医学院 临床检验诊断学教育部重点实验室 重庆市，400016 2遵义医学院附属医院 遵义，563003 摘要 目的 肺炎链球菌spd1672基因是本课题组前期筛选出的体内诱导基因，可能是新的毒力因子但功能未知，本研究的目的是研究该基因在肺炎链球菌中的生物学功能。方法 本研究在肺炎链球菌D39菌株、R6菌株中通过替代失活法构建spd1672基因敲除菌，并在生物信息学分析的基础上，比较基因缺失菌与野生菌在荚膜多糖合成、磷壁酸合成及细菌体外生长能力等方面的改变，从而鉴定其生物学功能。 结果 透视电子显微镜显示spd1672基因缺失菌与野生菌的荚膜多糖合成量无显著性差异，但C反应蛋白结合实验和western blot结果显示spd1672基因缺失菌磷壁酸合成能力较野生菌显著下降。此外，基因缺失菌在体外生长较野生菌体缓慢。结论 spd1672基因是肺炎链球菌的一种新毒力因子，影响肺炎链球菌磷壁酸合成和体外生长能力。 [关键词] 肺炎链球菌；spd1672基因；磷壁酸 In vivo inducible gene spd1672 decreased teichoic acid synthesis and bacterial growth of Streptococcus pneumoniae Huang Jian1, Zhang Yanqing1, Huang Meirong2,Wu Kaifeng1, Xu Wenchun1, Yin Yibing1, Wang Hong1# 1Key Laboratory Medical Diagnostics, Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China 2Zunyi Hospital affiliated to Zunyi Medical College, Zunyi , 563003, [Abstract] Objective As a vivo induced gene, Streptococcus pneumoniae spd1672 may be a novel virulence factor, but the function is unknown. The purpose of this study was to study its biological functions. Methods In this study, spd1672-deletion strains were generated by the method of alternative inactivation in Streptococcus pneumoniae D39, R6 strains. On the basis of bioinformatics analysis, the changes of capsular polysaccharide synthesis, teichoic acid synthesis and the in vitro growth in wild strains and spd1672-deletion strains were observed. Results Capsule polysaccharide of wild strains and spd1672-deletion strains did not obviously changes. However, the results of C-reaction protein binding test and western blot showed that the teichoic acid synthesis significantly reduced in spd1672-deletion strains when compared with wild strains. In addition, compared with wild strains, spd1672-deletion strains grow more slowly. Conclusion Spd1672 gene is a new virulenc