飞燕草素联合MA增敏抗HER-2阳性乳腺癌细胞效应1Delphinidin.PDFVIP

网络出版时间：2016-09-26 09:10:51 网络出版地址：/kcms/detail/11.1759.TS0910.044.html 1 飞燕草素联合 3-MA 增敏抗 HER-2 阳性乳腺癌细胞效应 陈静瑶，周杰，韩彬，李飞，朱彦锋，余小平* （成都医学院公共卫生系，四川成都 610500） 摘要:目的：研究飞燕草素(delphinidin, Dp) 联合自噬抑制剂 3- 甲基腺嘌呤 + (3-Methyladnine，3-MA)对 HER-2 乳腺癌细胞 MDA-MB-453 的效应。方法：用不 同浓度飞燕草素处理 MDA-MB-453 细胞，CCK-8 检测细胞增殖情况；荧光斑点 实验和 Weston Blot 实验检测飞燕草素对细胞自噬的诱导情况；Weston Blot 实验 检测 3-MA 对自噬的抑制状况，CCK-8 检测飞燕草素联合 3-MA 对细胞增殖状况 的影响。结果：飞燕草素能有效抑制 MDA-MB-453 细胞增殖，诱导 MDA-MB-453 细胞自噬并呈剂量依赖效应；3-MA 有效抑制 MDA-MB-453 细胞自噬，同时增强 + 自噬飞燕草素抗HER-2 乳腺癌细胞 MDA-MB-453 增殖效应。结论：飞燕草素联 合自噬抑制剂 3-MA，能促进飞燕草素抗HER-2+乳腺癌细胞 MDA-MB-453 增殖。 关键词：飞燕草素，乳腺癌，自噬，增敏 Delphinidin sensitizes anti-tumor effect to HER-2+ breast cancer cells by combining with 3-MA * CHEN Jing-yao，ZHOU Jie, HAN Bin, LI Fei, ZHU Yan-feng, YU Xiao-ping (Department of Public Health, Chengdu Medical College, Chengdu 610500, China) Abstract: Objective: To research the effect of delphinidin (Dp) combines with 3-Methyladnine (3-MA), inhibitor of autophagy, to HER (human epidermal growth factor receptor-2, HER-2) positive breast cancer cell MDA-MB-453. Methods: HER-2+ breast cancer cells MDA-MB-453 were treated by Dp. Proliferation of MDA-MB-453 cel