ELISA法和胶体金法检测血液抗―HIV结果对比分析.docVIP

ELISA法和胶体金法检测血液抗―HIV结果对比分析 　　【摘 要】目的：研究对比ELISA法与胶体金法检测血液抗-HIV的准确性和临床应用价值。方法：对2015年10月至2017年10月100份血样进行HIV抗体检验，所有血液样本均同时进行ELISA法与胶体金法检测；对比ELISA法与胶体金法检测血液抗-HIV的准确性和灵敏度。结果：ELISA法检出阳性23例，其中假阳性占4.55%；胶体金法检出阳性24例，其中假阳性占9.09%；ELISA法联合胶体金法检出阳性22例，检出率为100%；可见ELISA法检出抗-HIV阳性假阳性比胶体金法更低，二者联合检测则准确率更高。采用ELISA法检测，确诊为抗-HIV阳性最大稀释比例比胶体金法显著更大，差异有统计学意义（P0.05）。结论：ELISA法检测综合准确性优于胶体金法，但仍然有假阳性病例，因此在血清抗-HIV检测中，可联合采用ELISA法、胶体金法，二者互相补充以提高检测准确性。 　　【关键词】抗-HIV检测；ELISA法；胶体金法；准确性；灵敏度 　　Abstract Objective： To study the accuracy of the detection of blood ELISA method was compared with colloidal gold anti -HIV and clinical application. Methods： HIV antibody test from October 2015 to October 2017 of 100 blood samples， all blood samples were simultaneously detected by ELISA and colloidal gold method， showed positive samples sent to CDC confirmed by western blot. The detection of blood； the ELISA method was compared with colloidal gold anti -HIV accuracy and sensitivity. Results：ELISA was positive in 23 cases， including 4.55% false positives； colloidal gold were positive in 24 cases， the false positive rate was 9.09%； the ELISA method combined with colloidal gold were positive in 22 cases， the detection rate was 100%； the detection of anti -HIV positive false positive ratio of colloidal gold lower visible ELISA method， two joint detection is accurate a higher rate of. The maximum dilution ratio of anti -HIV positive diagnosed by ELISA method was significantly higher than colloidal gold method， the difference was statistically significant （P0.05）. Conclusion： ELISA comprehensive accuracy test is better than that of colloidal gold method， but there are still false positive cases， so the detection of serum anti -HIV， combined with ELISA method Colloidal gold method， the two complement each other in order to improve the accuracy of detection 　　Key words： Anti -HIV detection； ELISA method； colloidal gold method； accuracy； sensitivity 　　【中图分类号】R446.11 【文献标识码】A 【文章编号】1672-3783（2018）05-03-0-01 　　艾滋病全?Q为获