嗜冷普鲁兰酶产生菌NX-1的筛选及产酶条件优化.PDF

118 2015, Vol.36, No.01 食品科学 ※生物工程 嗜冷普鲁兰酶产生菌NX-1的筛选及产酶条件优化 1 1 1 2 1 1,* 康怀彬 ，肖天天，李净净，杨 桥 ，杨华田，尤晓颜 （1.河南科技大学食品与生物工程学院，河南 洛阳 471023 ；2. 中国水产科学研究院东海水产研究所，上海 200090 ） 摘  要：从南极海泥样品中分离得到一株产嗜冷普鲁兰酶的假交替单胞菌菌株（Pseudoalteromonas sp. ）NX-1 。以 菌株NX-1为研究对象，对菌株NX-1产嗜冷普鲁兰酶的酶学性质进行了初步研究，并通过单因素试验和正交试验对 其产嗜冷普鲁兰酶的最佳培养条件进行研究。结果显示，菌株NX-1所产嗜冷普鲁兰酶的最适作用温度为20 ℃，最 适作用pH值为7.0 ，在最适条件下，酶的半衰期约为120 min；菌株NX-1 的最适产酶条件为：培养温度20 ℃、接种 量1%、培养基各组分含量分别为蔗糖15 g/L、蛋白胨15 g/L、CaCl 0.5 g/L 、Na HPO 6 g/L 。优化后菌株产酶可达 2 2 4 25.172 U/mL，相比优化前提高25.1%。 关键词：嗜冷普鲁兰酶；假交替单胞菌；发酵；正交试验 Screening of Psychrophilic Strain NX-1, a Pullulanase-Producing Bacterium and Optimization of Culture Conditions for Pullulanase Production 1 1 1 2 1 1,* KANG Huaibin , XIAO Tiantian , LI Jingjing , YANG Qiao , YANG Huatian , YOU Xiaoyan (1. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China; 2. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China) Abstract: In the present study, a psychrophilic pullulanase-producing strain, Pseudoalteromonas sp. NX-1, was isolated from Antarctic marine sediment samples with optimum growth temperature of 20 ℃. The enzymatic properties of pullulanase from strain NX-1 were investigated. The medium composition and culture conditions for pullulanase production by NX-1 were optimized by single factor and orthogonal array experiments. The results showed the optimum catalytic pH of the pullulanase wa