一株拮抗真菌的蛇足石杉内生细菌分离鉴定及培养条件优化-微生物学报.PDF

微生物学通报 APR 20, 2008, 35(4): 512~518 Microbiology © 2008 by Institute of Microbiology, CAS tongbao@ 研究报告 一株拮抗真菌的蛇足石杉内生细菌分离 鉴定及培养条件优化 * 曾庆桂 朱 笃 颜日明 朱 飞 王志兴 ( 330022) 摘 要: 从蛇足石杉(Huperzia serrata)叶片中分离到一株对小麦赤霉(Fusarium graminearum)等多 种植物病原真菌有强拮抗作用的内生细菌 H-6 。通过形态和培养特性观察、生理生化实验及 16S rDNA 序列分析, 初步鉴定该菌属于伯克霍尔德属, 命名为Burkholderia sp. H-6 。同时还对该菌培 养条件进行了优化, 得出马铃薯浸出液中添加 2.5% 的甘露醇和0.1% NaNO3 有利于细胞生长和抑 菌活性的产生, 最适培养温度为28 ℃, 最佳初始pH4.0 。 关键词: 蛇足石杉, 内生细菌, 拮抗真菌活性, 鉴定 Isolation and Identification of Endophytic Bacteria H-6 with High Antifungal Activity from Huperzia serrata * ZENG Qing-Gui ZHU Du YAN Ri-Ming ZHU Fei WANG Zhi-Xing (College of Life Sciences, Jiangxi Normal University, Jiangxi Provincial Key Lab of Protection and Utilization of Subtropic Plant Resources, Nanchang 330022) Abstract: In this study, nearly 200 endophytic bacteria were isolated from different part of Huperzia serrata, over 60 bacterium with clear antifungal activity were selected from those cultures. Among them, strain H-6 exhibited the highest antifungal activity which was strongly inhibits the growth of many plant pathogenic fungi such as Sclerotinia scleroliorum, Fusarium graminearumt, Sclerotinia libertiana, Phytophthora capsici Leonia and Sesame fusarium wilt. According to the characteristics of morphology, physiology and biochem- istry tests and the comparison of 16S rDNA sequence, the strain H-6 was similar t