款冬叶柄愈伤组织培养和再生体系建立.docVIP

款冬叶柄愈伤组织培养和再生体系建立 　　[摘要]以款冬幼嫩叶柄为材料，研究植物生长调节剂对其离休培养与植株再生的影响，建立了款冬离体快繁技术体系。结果表明：款冬叶柄切段较理想的灭菌方法为75%乙醇浸泡30 s，转入饱和漂白粉上清液浸泡15 min；适合愈伤诱导的培养基为MS+6BA 30 mg?L-1+2，4D 20 mg?L-1，诱导率962%；在培养基MS+ZT 20 mg?L-1+NAA 03 mg?L-1上芽苗分化效果较好，分化率91%，平均芽数826个；较佳的不定芽增殖培养基为MS+KT 10 mg?L-1+IBA 03 mg?L-1，增殖倍?滴?1181，平均苗高49 cm；生根培养基为1/2MS+IBA 02 mg?L-1，生根数平均为568条，生根率为9522%以上；瓶苗移栽于河沙有机肥 3∶1的基质中生长良好，成活率达90%以上；大田试验表明：同等栽培条件下款冬组培株、栽培株和野生株在生长量与花粒产量等方面存在显著差异，以组培株生长量与花粒产量相对较高。组培品与野生品有效成分含量基本一致。 　　[关键词]款冬； 幼嫩叶柄； 愈伤组织诱导； 植株再生； 生长量； 有效成分测定 　　[Abstract]Young petiole of Tussilago farfara was used as the material to investigate the plant growth regulators which could influence in vitro culture and plant regeneration and to establish rapid propagation technique The ideal sterilization method was that young petiole of T farfara was sterilized with 75% ethanol for 30 s， and then transferred to saturated bleaching power supernatant for 15 min The suitable medium for callus induction was MS+6BA 30 mg?L-1+2，4D 20 mg?L-1 with 962% induction rate The seedlings had better differentiation with 91% differentiation rate and 826 buds on the medium containing MS+ZT 20 mg?L-1+NAA 03 mg?L-1 The preferred enrichment medium of adventitious bud was MS+KT 10 mg?L-1+IBA 03 mg?L-1with 1181 enrichment times and 49 cm seedling height The rooting medium included 1/2MS+IBA 02 mg?L-1 with the average number of rooting was 586 and the rooting rate was above 9522% The container seedlings can grow well and the survival rate was more than 90% when they were transplanted on the medium added with river sand and organic fertilizer with the ratio of 3∶1. The field experiments indicated that significant differences in increment and yield of pollen grains among the tissueculture， cultivation and wild type of T farfara under the same cultivation conditions The cultivated plants were relatively high on the increment and yield of pollen grainsThe active ingredient contnet of the tissue culture and the wild materials was basically the same. 　　[K