白细胞介素6基因型和桂北地区2型糖尿病肾病相关性研究.docVIP

白细胞介素6基因型和桂北地区2型糖尿病肾病相关性研究 　　摘要: 目的:探讨白细胞介素-6(IL-6)基因启动子-572 C/G多态性各等位基因及基因 型在2型糖尿病肾病患者中的分布频率,并分析其基因型与2型糖尿病肾病的相关性。方法: 采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测183例2型糖尿病肾病患者 ( 其中91例伴糖尿病肾病)及105例正常对照组IL-6基因多态性,包括IL-6基因启动子- 5 72C/G位点,同时采用酶联免疫吸附试验(ELISA)检测血清IL-6水平。结果:糖尿病肾病 组 IL-6血清水平显著高于糖尿病不伴肾病组和对照组(P0.01),IL-6基因多态性在两 组人群 中的分布差异存在显著性差异(P0.05),等位基因频率的相对风险分析发现,G等位 基因携带 者患糖尿病肾病的风险是C等位基因的1.618倍(OR=1.618,95%CI:1.082~2.418), 携带G 等位基因的糖尿病肾病患者IL-6血清水平显著高于不携带者(361.71±93.42 pg/ml VS343 .03±86.54 pg/ml, P0.05)。结论:IL-6基因启动子-572 C/G多态性与糖尿病 肾病的 发病具有相关性,其中G等位基因可能是糖尿病肾病发病的遗传易感基因,携带G等位基因的 个体可能通过促进IL-6的高度表达进而增加2型糖尿病肾病的发病风险。 　　关键词:白细胞介素-6; 糖尿病肾病;基因型 　　中图分类号: R587.1 文献标识码: A 文章编号: 1008-2409(2009)05-0801-04 　　 　　The genotype ofIL-6 gene in patients with type 2 diabetic nephropathy/CHEN Ju n-ning, YIN You-sheng, LI Xiao-li,et al.∥Department of Emergency, The Affil iated Hospital of Guilin Medical College, Guilin 541001, China 　　Abstract:Objective: To study the relationship the allele frequencies andgenotype distribution of interleukin(IL-6)gene promoter -572C/G polymorphismi n Chinese patients with type 2 diabetes mellitus and diabetic nephropathy(DN),A nd analyze association the serum levels and genotype of IL-6 with type 2 diab e tic nephropathy.Methods: The IL-6 gene promoter -572C/G polymorphism were det e cted by polymerase chain reaction-restriction fragment length polymorphism(PCR - RFLP)analysis among 183 patients with type 2 diabetes mellitus and 105 healthycontrols. The serum level of IL-6 was determined by enzyme-linked immunos or bent assay(ELISA). Results: The diabetic nephropathy group showed significantlyhigher serum levels of IL-6 than that of control group(P0.01). There was s ignificant di fference in frequencies of allele and genotype in -572C/G polymorphism between t ype 2 diabetic nephropathy group and control groups, respectively. The relativerisk suffered from type 2 diabetic nephropathyofGallele was 1.618 times of t he C allele(OR=1.618,95%CI:1.082~2.418), the serum IL-6