灰毡毛忍冬种子质量检验方法和分级标准研究.docVIP

灰毡毛忍冬种子质量检验方法和分级标准研究 　　[摘要]参照《中国农作物种子检验规程》，研究扦样、净度、千粒重、真实性、水分、生活力、相对电导率、发芽等灰毡毛忍冬种子质量检验方法；对38份不同来源的种子做质量检验，采用K均值聚类制定质量分级标准。结果表明：灰毡毛忍冬种子最少试验量为75 g，过20目筛进行净度分析，五百粒法测定千粒重，种子粉碎高恒温（130±2）℃烘干3 h测定水分含量，01%TTC溶液25 ℃避光染色5 h测定生活力，10 g种子在25 ℃超纯水中浸泡12 h测定相对电导率，4 ℃下沙藏层积80 d后置于15 ℃光照纸床进行发芽试验；不同来源种子按质量分为3个等级，初步制定了种子质量分级标准。生产上建议使用Ⅰ级和Ⅱ级种子。 　　[关键词]灰毡毛忍冬；种子；质量检验；分级标准 　　[Abstract] Referring to the rules for agricultural seed testing （GB/T issued by China， the test of sampling， purity， thousand seed weight， moisture， viability， relative conductivity and germination rate had been studied for seed quality test methods of Lonicera macranthoides. The seed quality from 38 different collection areas was measured to establish quality classification standard by Kmeans clustering The results showed that at least 75 g seeds should be sampled， and passed 20mesh sieve for purity analysisThe 500seed method used to measure thousand seed weight The moisture was determined by crushed seeds dried in high temperature （130±2 ℃） for 3 hThe viability determined by 25 ℃ 01% TTC stained 5h in dark 10 g seeds soaked in 50 ml ultra pure water in 25 ℃ for 12 hours to determine the relative conductivity The seed by 4 ℃stratification for 80 days were cultured on paper at 15 ℃. Quality of the seeds from different areas was divided into three grades The primary seed quality classification standard was establishedThe I grade and II grade were recommend use in production 　　[Key words]Lonicera macranthoides； seed； quality test methods； quality classification standard 　　doi：10.4268/cjcm 　　灰毡毛忍冬Lonicera macranthoides HandMazz为忍冬科忍冬属多年生藤本植物，是我国重要大宗药材山银花的基原植物之一，花蕾含有有机酸、黄酮类、皂苷类等化学成分，具有清热解毒，凉散风寒的功效，社会需求量极高[12]。灰毡毛忍冬生态适应性强、产量高、采摘方便、绿原酸含量高，在重庆、湖南、贵州、四川等地广泛种植[3]。 　　灰毡毛忍冬实生苗繁育成本低、根系活性强，是采用嫁接技术繁育良种的优质砧木材料，生产上广泛应用[46]。但不同产地和成熟程度的灰毡毛忍冬种子质量差异悬殊，严重制约实生苗的生产，迫切需要建立灰毡毛忍冬种子质量检验和分级标准。目前对灰毡毛忍冬的研究主要集中在化学成分、药材质量、栽培技术等方面，未见种子质量相关研究[2，4，612]。本文开展灰毡毛忍冬种子质量检测方法的研究，测定不同来源灰毡毛忍冬种子质量，初步建立了灰毡毛忍冬种子质量评价体系和分级标准。 　　1材料与方法 　　11