嘎木朱尔妇康片剂局部用药的毒性研究.docVIP

嘎木朱尔妇康片剂局部用药的毒性研究 2OO9年8月第8期中国民族医药杂志53 刺老苞根皮黄酮化合物处理的各组细胞RANK基因表达水 平均有所上调,且呈现一定的量效关系.这些结果均初步 表明刺老苞根皮黄酮化合物在体外可以有效地抑制破骨细 胞的形成与分化,有助于延缓骨吸收过程,但其中作用机制 还需要进一步深入的研究. 参考文献 [1]邵兴,赵敬华.浅析自然地理环境与土家族医药学的关 系[J].中国民族民间医药,2007,11(3):58—60. [2]方志先,赵晖,赵敬华.土家族药物志[M].第一版,中国 医药科技出版社,2o07:830—835., [3]李永恕.土家族民间医药治疗骨病骨伤经验[J].中国民 族医药杂志,2001,7(3):57—59. [4]马静波.骨质疏松症的治疗和预防概述[J].医师进修杂 志,2005,28(5):9一l1. [5)YasudaH.ShimaN.Anovelmolecularmechanismmodulating osteoclastdifferentiatiionandfunction(J].Bone,1999,25(6): 109—133. [6]KumedaY,InabaM,NishizawaY.Secondaryosteoporosisand itstreatmentdiabetesMeUitus[J].NipponRinsho,1998,56 (6):1579—1582. 2009年5月6日收稿 EffectofAraliaechinocaulisHand.Mazzflavonoidsonthedifferentiation ofosteoclastinducedfrombonemarrowinmice PEILingpeng,YInXia2,DongFuhui (1.CentralMINZUUniversity,InstituteofMinarityTraditionalMedicineResearch,Beijing100081,China;2.China AcademyofChineseMedicalSciences,WangjingHospital,InstituteofOrthopaedicsandTraumatology) AbstractObjective:EffectofAraliaechinocaulisH.and.Mazzflavonoidsonthedifferentiationofosteoelastinduced frombonemarrowvitro.Methods:Mononuclearcellsofmacebone/nalTOWwereincubatedwitI1DMEMcontainingmac. rephagecolonystimulatingfactor(M—CSF)andreceptoractivatorof/W一曲ligand(RAN~).111eculturewas treatedwithAraliaechinocaulisHand.Mazzflavon6idsofdifferentconcentrations(10～mol?L,10moJ.L,J0～m0卜 L).Onthe7d,theculturecellswerefixedandwerestainedfortartate—resistantacidphossphatase(TRAP).Thefor— mationofosteoelastsWasquantifiedbycountingthenumberofTRAPmuhinuelearceils.11activityandtheex— pressionofreceptoractivatorofNF—KB(RANK)mRNA.Results:TheappearanceofreducedmiceosteoclastsWas帅一 ica1.Comparedwiththecontrolgroup.thenumberofTRAPmuhinuclearcellsandTRAPactivityofAraliaechino. caulisHand.Mazzflavonoidsmiddle—dosegroupandhigh—dosegroupsignificantlydecreased(Plt;0.05).Butthe expressionofRANKmRNAsignificantlyincreased(Plt;0.05)bytreatedindifferentdoseAraliaechinocaulisHand. Mazzflavonoids.Conciusion:AraliaechinocaulisHand.Mazzflavonoidscani