二甲双胍治疗多囊卵巢综合征机制的研究.docVIP

二甲双胍治疗多囊卵巢综合征机制的研究 　　[摘要] 目的 探?二甲双胍在治疗多囊卵巢综合征（PCOS）中对Treg细胞和Th17细胞的作用及其机制。 方法 雌性Wistar大鼠45只，来曲唑灌胃建立PCOS大鼠模型，随机抽取5只确立成模后，按完全随机分组原则分为二甲双胍组[300 mg/（kg?d）]、达英组[180 mg/（kg?d）]、二甲双胍[300 mg/（kg?d）]与达英[180 mg（kg?d）]联合治疗组及模型组，每组10只；另选取10只大鼠作为对照组。成模后，各治疗组采用相应药物灌胃治疗，模型组给予等量生理盐水，均连续处理28 d，观察各组大鼠每周体质量变化。苏木精-伊红（HE）染色法观察卵巢组织学变化，流式细胞术检测脾脏中Treg细胞和Th17细胞数量及表型的改变，ELISA法检测血清中IL-6和TGF-β水平，RT-PCR法测定卵巢组织转录因子Foxp3、ROR-γt的表达水平。 结果 模型组大鼠体质量、脾脏中Treg细胞、Th17细胞数量以及Treg/Th17比例、血清中IL-6及TGF-β水平以及卵巢组织中转录因子Foxp3的相对表达量显著高于对照组（P 0.05）；二甲双胍组、达英组及联合用药组囊性扩张卵泡较模型组明显减少；二甲双胍组脾脏Treg细胞、Th17细胞、血清IL-6水平及卵巢组织中转录因子ROR-γt的表达水平较模型组显著升高（P 0.05），达英组及联合用药组脾脏Th17细胞、血清IL-6水平与模型组相比显著降低（P 0.05），而卵巢组织中转录因子Foxp3的表达水平较模型组显著升高（P 0.05）。 结论 二甲双胍治疗PCOS的作用机制可能是通过影响血清细胞因子IL-6表达水平，调节Treg、Th17细胞的数量及比例，进而改善卵巢组织微环境。 　　[关键词] 多囊卵巢综合征；Treg；Th17；二甲双胍 　　[中图分类号] R392.1 [文献标识码] A [文章编号] 1673-7210（2017）11（c）-0017-05 　　Study on the mechanism of Metformin in the treatment of polycystic ovary syndrome 　　LU Chenyu QIN Peigang DU Hanyu WU Haixia Aihemaiti?Guliyaer AI Lixia XU Qi 　　Basic Medical College， Xinjiang Medical University， Xinjiang Uygur Autonomous Region， Urumqi 830011， China 　　[Abstract] Objective To investigate the therapeutic mechanism of Metformin on Treg cells and Th17 cells in polycystic ovary syndrome （PCOS）. Methods 45 female Wister rats were gavaged by Letrozole in order to develop PCOS， and randomly chosen 5 rats to confirm the establishment of model. Then rats were divided into Metformin group [300 mg/（kg?d）]， Diane group [180 mg/（kg?d）]， Metformin [300 mg/（kg?d）] combined with Diane [180mg/（kg?d）] group and model group， randomly， each group had 10 rats. The other 10 female rats were arranged as control group. PCOS rats were given corresponding drugs， while control group were administrated with physiological saline， the treatment period of all groups were 28 days. The weight changes of rats weekly were observed. Ovarian histological changes were examined by Yihong-hematoxylin staining （HE）. Flow cytometry was applie