（毕业学术论文设计）-有除草活性的放线菌菌株1-10固体发酵的研究.docVIP

精品 精品 有除草活性的放线菌菌株1-10固体发酵的研究 精品 精品 摘 要 本实验通过配制固体培养基，对有除草活性的放线菌菌株1-10进行固体发酵。固态发酵是没有或只有少量游离水存在，在具有可以满足微生物生长代谢的一定湿度的固态营养基质中进行的微生物发酵过程。固体发酵具有操作简便、能耗低、发酵过程容易控制、对无菌要求相对较低、不易发生大面积的污染等优点。固体基质不仅起到一个提供碳源、氮源、水分、无机物和其他营养物的作用，同时也为微生物的生长提供了一个固定的位置，整个发酵过程跟微生物在自然环境中所发生的微生物反应相似。通过对菌株进行固体发酵，然后用发酵悬浮液对草进行涂抹处理，利用固体发酵悬浮液对草进行处理后测其株防效。从第1-12d得到的结果依次为：0、3.15%、7.28%、16.92%、28.33%、47.54%、86.82%、59.41%、23.90%、12.42%、6.35%、1.72%，所以第7d为最佳发酵时间，株防效为86.82%。 关键词：固体发酵；发酵时间；除草活性 ABSTRACT This experiment through the solid culture medium configuration, conducted solid fermentation to the actinomycetes strains 1-10 which had herbicidal activity. There is no or only small amount of free water in the solid fermentation, which is conducted in the solid-state nutrition matrix that can meet the suitable humidity for the microbes to grow up. Solid fermentation has many advantages such as easy to control, low energy consumption, easy to handle the fermentation process, low requirements to the sterile condition, not easy to pollute large area and so on. Not only dose Solid matrix provide a carbon source and nitrogen source, water and inorganic nutrients and other, but it also provides a fixed location for microbial growth, the whole of the microbial fermentation process is similar to the microbial reactions that occur in the natural environment. Through solid state fermentation to the strains, then daub the grass with fermentation suspension, use the solid fermentation suspension daub the grass and test the strains control effect. The results as follows: 0, 3.1%, 28.3% 7.2%, 16.9%, 47.5%, 86.8%, 59.4%, 12.4%, 6.3%, 23.9%, 12.4%, 6.35%, 1.7%. The seventh day is the best fermentation time. Key words: Solid fermentation；Fermentation time; Herbicidal activity 目 录 1.前言 1 1.1杂草的危害 1 1.2除草剂研究进展 1 2.材料与方法 3 2.1实验仪器材料及常用的培养基 3 2.2实验步骤 3 2.3菌种的保存与鉴定 4 3.结果与分析 5 3.1固体发酵 5 3.2测定最适发酵时间 5 3.3菌种的鉴定 6 4.讨论 7 4.1固体发酵条件的控制 7 4.2固体发酵的方法有利于放线菌的发酵 7 5.结论 8 5.1固体培养基的方法适合生长 8 5.2掌握了最佳发酵时间 8 参考文献 9 致谢 10 精品 精品 1.前言 1.1 杂草的危害 杂草是