儿童难治性肺炎支原体肺炎免疫机制的探讨.docVIP

儿童难治性肺炎支原体肺炎免疫机制的探讨 　　[摘要] 目的 探讨儿童难治性支原体肺炎的免疫功能变化。 方法 选择2014年1月～2015年5月在河北省儿童医院治疗的难治性支原体肺炎患儿47例（难治性组）、普通支原体肺炎患儿50例（普通组）以及健康体检儿童50例（对照组）为研究对象。采用流式细胞仪以及免疫透射比浊法检测所有研究对象T淋巴细胞亚群以及外周血免疫球蛋白水平，分析儿童难治性肺炎支原体肺炎的免疫机制。 结果 难治性组患儿血清IgG、IgM、CD8+、CD19+均显著高于对照组，CD3+、CD4+、CD4+/CD8+、CD16+、CD56+均显著低于对照组，差异均有高度统计学意义（P 0.05）。 结论 体液免疫及细胞免疫均参与了儿童难治性肺炎支原体肺炎的发病，患儿存在免疫功能紊乱，并且持续时间较长。 　　[关键词] 儿童难治性肺炎支原体肺炎；免疫机制；T淋巴细胞亚群；外周血免疫球蛋白 　　[中图分类号] R725.6 [文献标识码] A [文章编号] 1673-7210（2016）02（c）-0107-04 　　Discussion of immune mechanism of children refractory mycoplasma pneumoniae pneumonia 　　NIU Bo1 CHI Yuepeng2 SHUAI Jinfeng1 YANG Huirong1 HUANG Kunling1 CAO Lijie1 LIU Jianhua1 　　1.The Second Ward of Department of Respiration， Childrens Hospital of Hebei Province， Hebei Province， Shijiazhuang 050031， China； 2. Department of Tuberculosis， the Chest Hospital of Hebei Province， Hebei Province， Shijiazhuang 050041， China 　　[Abstract] Objective To discuss the immune mechanism of children refractory mycoplasma pneumoniae pneumonia （RMPP）. Methods From Jan 2014 to May 2015， 47 cases with RMPP （RMPP group）， 50 cases with MPP （MPP group） and 50 healthy children （control group） in Childrens Hospital of Hebei Province were selected as study subjects. Peripheral blood T lymphocyte subsets and immunoglobulin levels were detected with flow cytometry and immune turbidimetry， and the immune mechanism of children refractory mycoplasma pneumoniae pneumonia was analyzed. Results Serum levels of IgG， IgM， CD8+， CD19+ of the RMPP group were higher than those of the control group， and serum levels of CD3+， CD4+， CD4+/CD8+， CD16+ CD56+ were lower than those of the control group， the differences were statistically significant （P 0.05）. Conclusion Both humoral immunity and cell-mediated immunity are involved in the pathogenesis of RMPP. Children with RMPP combine with the presence of immune dysfunction， and long duration. 　　[Key words] Refractory mycoplasma pneumoniae pneumonia； Immune mechanism； T-lymphocyte subsets； Per