干旱胁迫下大豆幼苗根系基因的表达谱分析-中国农业科学.PDFVIP

中国农业科学 2010,43(22):4579-4586 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2010.22.004 干旱胁迫下大豆幼苗根系基因的表达谱分析 宋雯雯，李文滨，韩 雪，高慕娟，王继安 （东北农业大学农学院/大豆生物学省部共建教育部重点实验室，哈尔滨 150030 ） 摘要： 【目的】了解干旱胁迫下大豆幼苗根系的抗旱机制。【方法】利用基因芯片技术，分析在不同干旱胁 迫时间下强抗旱品种晋豆 23 幼苗根部基因的表达情况。【结果】获得了 61 171 个大豆根系基因的差异表达动态 图谱。在不同干旱胁迫时间下根部基因的表达发生变化，3 个时间点下，下调表达的基因数量均多于上调表达的 基因数量。同时对杂交数据进行多种聚类分析。利用实时荧光定量 PCR 验证 4 个基因在干旱胁迫条件下的差异表 达,其结果和芯片杂交分析的结果基本一致。【结论】初步建立了大豆幼苗根系干旱胁迫应答基因的动态表达谱， 并通过其动态表达了解植物与逆境的互作机制，比较全面地获得了干旱胁迫应答基因，从而更完整地揭示了大豆 干旱胁迫应答基因的表达情况。 关键词：大豆幼苗根系；干旱胁迫；基因芯片；实时荧光定量PCR Analysis of Gene Expression Profiles in Soybean Roots Under Drought Stress SONG Wen-wen, LI Wen-bin, HAN Xue, GAO Mu-juan, WANG Ji-an (College of Agronomy, Northeast Agricultural University/Chinese Education Ministry’s and Provincial Key Laboratory of Soybean Biology, Harbin 150030) Abstract: 【Objective 】 The objective of this experiment is to study the mechanism of drought resistance in soybean seedling roots. 【Method 】 The gene expression of drought-tolerant soybean roots was analyzed under different times of drought stress by cDNA microarray chip. 【Result 】 The expression profile of 61 171 genes was obtained. Great changes took place in soybean roots under drought stress and the number of induced genes was more than that of repressed genes. The differential expressions of four genes were confirmed by real-time quantitative PCR and the results indicated that the expression changes of these genes were generally consistent with the results of gene chip. The clustering analysis of hybridization was also carried out to indicate gene expression under drought stres