藏药川西獐牙菜醇提物正丁醇萃取部位化学成分的研究-植物学专业论文.docxVIP

万方数据 万方数据 摘要 川西獐牙菜（Swertia mussotil Franch ）属于龙胆科（Gentianaceae）獐 牙菜属(Swertia)植物，是藏药“蒂达”的原植物，全草可入药，且用药历史久 远。最近几年，对其进行药理方面的研究，结果发现川西獐牙菜的煎剂、复方药 等对治疗肝炎、心血管疾病以及免疫调节等方面有确切疗效。为了提供川西獐牙 菜在治疗肝炎等方面的药效物质基础，本实验对川西獐牙菜醇提物的正丁醇萃取 部位的成分进行了提取以及分离鉴定。 本实验通过甲醇回流提取 3 次，料液比为 1:6，每次 2 小时，减压浓缩。然 后加入蒸馏水与浸膏混悬（液膏比 2:1），依次使用石油醚、三氯甲烷和正丁醇 萃取分段。 实验运用柱色谱法及薄层色谱法以及重结晶等方法共分离得到了 7 种组分， 经过核磁共振技术的鉴定，分别为：异荭草苷、芒果苷、7-O-[β-D-吡喃鼠李糖 -(1-2)-β-D-吡喃木糖]-1,8-二羟基-3-甲氧基口山酮、齐墩果酸、1-O-[β-D-吡喃葡萄 糖-(1-6)-β-D-吡喃葡萄糖]-8-羟基-3,7-二甲氧基口山酮、bellidifolin 和 1-羟基-3,5,8- 三甲氧基口山酮等。 关键词：川西獐牙菜，柱色谱，口山酮 I Abstact Swertia mussotil Franch belongs to Swertia of Gentianaceae. As the basic“Dida”of Tibetan medicine the whole part of which has been directly used in clinical curative for a long history. Recently pharmacological research on it has found that its decoction and the compounds have clear curative effect to hepatitis ， vasodilation and immunoregulation. In order to provide pharmacodynamic material bases on identify what the elementary elements are in remedying disease such as hepatitis. This experiment makes extraction and separate analysis about N-butanol extract of Swertia mussotil Franch. We got the Extract of Swertia mussotil Franch by methanol，the best conditions： solid-liquid ratio of 1：6，refluxing extraction for 2 hours，extraction times 3 times. Then suspended the dry extractum with distilled water and extract with Petroleum ether、Chloroform、Butyl alcohol by order. Six elements has been got by applying column chromatography and，TLC， recrystallization and indentified through NMR，seven structures of the compounds were as Isoorientin ， Mangiferin ， 7-O-[β-D- Pyran-Rhamnopyranosyl -(1-2)-β-D-Xylopyranose]-1,8-hydroxy-3-methoxy xanthone 、 oleanolic acid ， 1-O-[β-D- Glucopyranose-(1-6)-β-D-Glucopyranose]-8- hydroxy-3,7-dimethoxy xanthone，bellidifolin，1-hydroxy-3,5,8-trimethoxy xanthone. Key words ： Swertia mussotil Franch ， column chro