藏药苦空确屯卡察尔保肝作用研究及机制探讨药理学专业论文.docxVIP

成都中医药大学硕士学位论文 成都中医药大学硕士学位论文 PAGE PAGE 10 用；②通过促进胆汁分泌、保护胆管上皮细胞、减少炎症发生起到退黄利胆 的作用；③通过增强单核巨噬细胞的吞噬作用起到增强非特异性免疫功能的 作用。 关键词：苦空确屯卡察尔（ZYJN） 保肝 利胆 退黄 α-SMA TGF-β1 MMP1 ABSTRACT Object: To study the hepatic-protective effects and mechanism of “kukongquet- unkachaer”(ZYJN) which was the empirical formula of tibetan medicine and used for various kinds of acute hepatitis, chronic hepatitis clinically. Metherd: ①D-Galn was given to the mouse by intraperitoneal injection to establish acute liver injury model. The hepatosomatic index of the mouse、the alanine aminotransferase(ALT), aspartate aminotransferase (AST) levels in serum were all detected, and the liver pathological injury were observed after HE staining. CCL4 was given to the rat by subcutaneous injection to establish chronic liver injury models. The ALT,AST,total protein(TP),albumin(ALB),total bilirubin(T-BIL), laminin(LN), Hyaluronic(HA), levels in serum, hepatosomatic index and the malonaldehyde(MDA), hydroxyproline(Hyp) contents in liver homogenate were determined, the liver pathological injury was observed after HE staining and immunohistochemistry staining. All above were to research the protective effect of ZYJN on the liver. A pipe was intubated into the common bile duct of the rat, and bile flow of the rat was observed to research the affection of ZYJN on the bile flow; α-isothiocyanate(ANIT) was given to the mouse by intraperitoneal injection to made cholestatic jaundice, and the effects of ZYJN on jaundice-relieving was observed. Cyclophosphamide was given to the mouse by intraperitoneal injection to establish hyp-immune models, the effects of ZYJN on immune function was observed. Results ： ①In mouse acute liver injury models induced by D-Galn,ZYJN significantly decreased ALT,AST levels in serum and improved the morphological changes; In rat chronic liver injury models induced by CCL4, ZYJN significantly decreased ALT,AST,LN,HA levels in serum,MDA,Hyp contents in liver homogenate， increased T