侧脑室注射P63抗体对大鼠脑缺血后P63和P53表达及神经元凋亡的影响-麻醉学专业论文.docxVIP

PAGE PAGE 10 结论： 1.大鼠脑缺血后海马 CA1 区 P63 与 P53 表达均明显增加，侧脑室注射 P63 抗体能减少 P63 表达但对 P53 表达无明显影响。 2.侧脑室注射 P63 抗体能减少大鼠脑缺血后的神经元凋亡，改善神经 行为学评分而具有神经保护作用。 3.大鼠脑缺血后海马 CA1 区 P63 表达、P53 表达和神经元凋亡三者均 呈正相关。 关键词：p63；侧脑室注射 ；脑缺血；神经元凋亡；p53；大鼠 The effects of intracerebroventricular injection of P63 antibody on P63 and P53 expression and neuronal apoptosis after cerebral ischemia in rats Abstract Objective: To observe the effects of intracerebroventricular injection of P63 antibody on P63 and P53 expression, neuronal apoptosis in hippocampal CA1 area after global cerebral ischemia in rats, and to explore the effects of p63 on P63 and P53 expression and neuronal apoptosis after cerebral ischemia and anoxia, and to provide new theoretical basis for prevention and cure of neuronal apoptosis after cerebral ischemia and anoxia. Methods: According to random number table, 75 male SD rats were randomly divided equally into three groups: group A, B and C. The rats in group A were received artificial cerebrospinal fluid injected into lateral ventricle without cerebral ischemia; the rats in group B were received artificial cerebrospinal fluid injected into lateral ventricle and cerebral ischemia; the rats in group C were received P63 antibody injected into lateral ventricle and cerebral ischemia . The neurological behavior scores of rats in three groups were evaluated before anesthesia, and the rat with 16-20 scores was selected into this study. All rats were evaluated espectively neuroethology at 6h, 12h, 24h, 48h, 72h after operation. Under general anesthesia, all rats were executed and brain tissues were collected after perfusion with 4% formaldehyde polymerisatum. The neuronal densities were determined by HE, neuronal apoptosis indexes were determined by TUNEL, P63 and P53 expression were detected by immunohistochemistry (IHC) and semi-quantitative analysis in hippocampal CA1 area. Results: 1.Animal model In the study, 7 rats died: 5 rats died from cardiac arrest and apnea, and 2 rats died after