多功能抗癌纳米药物的设计合成及其在癌症治疗中的应用研究-材料科学与工程专业论文.docxVIP

II II 中文摘要 多功能抗癌纳米药物的设计合成及其在癌症治疗中的应用研究 功能纳米药物颗粒，结合了以上所有功能于单个纳米颗粒中，有望为癌症治疗的 发展提供新思路，并为临床应用提供依据。 关键词：纳米药物，长径比效应，多功能，靶向输送，诊疗一体化 作 者：田柏顺 指导老师：张秀娟 PAGE IV PAGE IV 多功能抗癌纳米药物的设计合成及其在癌症治疗中的应用研究 英文摘要 Synthesis of multifunctional anticancer nanodrugs and their applications in cancer therapy Abstract In this thesis, we have studied the aspect ratio (AR) effect of drug nanocrystals and developed a multifunction nanoparticle for cancer therapy. The results are listed as follows: HCPT NCs with four different ARs were controllably developed, which exhibited a strong AR dependent on cellular internalizing efficiency, uptake mechanisms, biodistributions as well as in vitro and in vivo anticancer efficiency. Shorter HCPT NCs (AR=1.3) showed obviously enhanced internalization rates than the longer NCs (AR=2.0, 3.2 and 4.0), which maybe a benefit from the fact that shorter NCs possess much larger surface areas in contact with the cell membrane than the longer ones. In vitro cytotoxicity experiments showed that shorter NCs exhibited a higher toxicity. In vivo experiments showed that NCs with AR=4.0 had a shortest half-life blood circulation time and were more easily captured by the liver, while NCs with the smallest AR possessed the highest therapeutic efficacy with appreciably less weight loss. These results indicate that aspect ratio is an important parameter which have a significantly effect on drug efficiency, and which would assist in the future design of drug nanocrystals in bioapplications. We have synthesized MFALPM nanoparticles (NPs) based on methotrexate (MTX) nanocore, Fe3O4 magnetic (NPs), gold nanoshells and LA-PEG-MTX coated as a multifunctional platform to combine magnetic and active-targeted drug delivery, MR imaging, NIR light-triggered drug release, and photothermal therapy into one system simultaneously. The resulting MFALPM NPs display surface plasmon absorbance in the NIR region, thus exhibiting an NIR-induced temperature elevation and an NIR 英文摘要 多功能抗