侧壁沟通式十字交叉微圆通道装置中明胶微液滴的生成及操纵生物学专业论文.docxVIP

重庆大学硕士学位论文英文摘要 重庆大学硕士学位论文 英文摘要 PAGE PAGE IV ABSTRACT Microfluidics is becoming more and more important as an interdisciplinary technique．Microfluidic chip droplets generation technology which plays an inportant role in biological miniaturization experiment, becomea the rapid dvelopment direction recent years. In this thesis, based on polydimethylsiloxane (PDMS) microfilament molding method, through-hole-on-wall of crossed round microchannels device are used to generate gelatin micro-droplets. First, we report a microfilament molding method to build through-hole-on-wall of crossed round microchannels device, and integrate microfilament molding method, we discuss the possibility to produce a new microfluidic device of configuration microstructurethe on polymethyl methacrylate methyl ester (PMMA) substrate. Then, based-on crossed microchannel, we constructed a T-type through-hole-on-wall of crossed round microchannel through blocking one side of the cross-hole. Secondly, the conditions micro-droplets generated through controlling gas / liquid two-phase and liquid / liquid two-phase in the above device are consided, focusing on the gelatin droplets on condition of liquid / liquid two-phase. The results show that the required minimum flow ratio of dispersed phase to continuous phase has relation to the type of oil phase, the channel diameter and the concentration of gelatin solution. ① When the microchannel diameter is 150μm and the dispersed phase of gelatin solution concentration is 2%, the minimum flow ratio of the silicone oil and edible oil is 75 and 15 respectively. ② When edible oil is continuous phase and gelatin solution of 2% concentration is dispersed phase, gelatin droplets genration require the minimum flow ratio is 15, 22.5, 27.5, 36.5, 51 in the microchannel with the diameter of 150μm, 100μm, 80μm, 60μm, 40μm. ③ When the diameter of the microchannel is 150μm and made the edible oil as the continuous phase, the gelatin solution has the concentration of 1