多壁碳纳米管体内应用对小鼠免疫应答和白血病发生发展影响-生物化学与分子生物学专业论文.docxVIP

II II 中文摘要 多壁碳纳米管体内应用对小鼠免疫应答和白血病发生发展的影响 6）不同的碳纳米管注射次数下，白血病小鼠的存活期不同； 7）碳纳米管能够促进巨噬细胞 RAW264.7 释放促炎因子。 结论： 碳纳米管能够促进免疫状态正常小鼠的白血病发生进程，而对免疫抑制和免 疫缺陷小鼠的白血病发生进程没有明显影响。该现象可能与促炎因子的释放，从 而导致慢性炎症有关。 关键词：白血病 小鼠模型 多壁碳纳米管 免疫功能 作 者：连雪琪 指导老师：尹斌 PAGE PAGE IV 多壁碳纳米管体内应用对小鼠免疫应答和白血病发生发展的影响 英文摘要 The effect of in vivo use of multi-wall carbon nanotubes on murine immune response and leukemogenesis Abstract Purpose: To explore the biological safety of carbon nanotubes by studying the impact of carbon nanotubes on mouse leukemia model, the influence on leukemogenesis after immune function changes, and the relevant molecular mechanisms. Methods: Preparation of oxidized water-soluble multi-walled carbon nanotubes; Establishment of mouse leukemia model; Subcutaneous injection of carbon nanotubes into immuno-competent mice to observe the incidence, record the survival time, and compare the survival rate and reveal the effects of carbon nanotubes on leukemia; Detection by FACS of the subtype of leukemia induced by MOL4070 virus; Subcutaneous injection of carbon nanotubes to rapamycin-suppressed and immune-deficient mice to reveal the relationship among carbon nanotubes, immune response and leukemogenesis; Detection of the levels of pro-inflammation cytokines in the peripheral serum of mice by ELISA; Detection of the levels of pro-inflammation cytokines in the supernatant of RAW264.7 treated with carbon nanotubes by ELISA. Results: Successful preparation of the oxidized water-soluble multi-walled carbon nanotubes; Successful establishment of the mouse leukemia model; The leukemia type induced by MOL4070 virus was mostly myeloid leukemia,followed by lymphoid and mixed leukemia; The survival rates of leukemia mice injected with carbon nanotubes were 英文摘要 多壁碳纳米管体内应用对小鼠免疫应答和白血病发生发展的影响 different in leukemia mice under different immune status; The levels of pro-inflammation cytokines injected with carbon nanotubes were different in leukemia mice under different immune status; The survival rates of leuk