多糖基生物材料制备核壳纳米粒及其对药物的pH敏感释放研究-材料学专业论文.docxVIP

万方数据 万方数据 摘要 目前，由于具备在不同环境刺激下对抗癌药物，标记染料的控制释放，以及 其本身固有的生物相容性，基于多糖类材料制备的多功能纳米粒备受研究者青睐。 本文主要利用壳聚糖（Cs）和聚乙烯醇磷酸酯（PPVA）作为静电多层材料，借 助层层自组装技术，交替沉积在3-氨丙基三乙氧基硅烷改性修饰的二氧化硅球表 面制备了多功能纳米粒。通过透射电子显微镜分析和粒径分布表征，证明了纳米 粒具备良好的球形形貌。7-羟基香豆素和罗丹明B作为二种模拟药物分别装载在 了核层和壳层之中。通过激光共聚焦显微镜证明了核壳结构，进一步验证了药物 的分布位置。通过研究不同pH环境下，7-羟基香豆素和罗丹明B的共释放行为， 表明了SiO2(PPVA/Cs)n这种多功能核壳纳米粒在药物递送领域具有崭新的应用 前景。 另一方面，我们还利用预凝胶方法制备了多糖基（果胶，卡拉胶）羟基磷灰 石的核壳纳米粒。通过固定多糖结构单元与钙离子摩尔比，调节羟基磷灰石的钙 磷比，研究了多糖分子与羟基磷灰石的相互作用，通过透射电镜观察了纳米粒的 形貌特征，最终确定了以羟基磷灰石晶体为核，阴离子多糖为壳的无机-有机杂 化核-壳纳米粒的制备方法。通过在纳米粒表面进行静电层层自组装得到了核壳 纳米杂化材料，材料不仅显示出了果胶和卡拉胶的生物相容性，而且具备羟基磷 灰石的pH可降解性。最后，通过研究两种纳米粒在不同pH下对罗丹明6G的释放 行为，得出结论：在酸性条件下由于羟基磷灰石的溶解导致水溶性药物罗丹明6G 从纳米粒中大量释放。研究结果表明，基于生物多糖形成的纳米核壳材料在生物 医药领域具有潜在的研究价值。 关键词：核壳结构；层层自组装；pH 敏感性；羟基磷灰石；生物多糖； 药物递送 ABSTRACT Recently, controlled release nanosystems based on a wide range of biopolysac- charides materials, capable of selectively releasing cargo, that is, anticancer drugs and labeling dyes, under different external stimuli, have demonstrated their viability for use in a variety of biological applications. In this work, bilayers of chitosan (Cs) and phosphorylated polyvinyl alcohol (PPVA) were sequentially deposited on 3-aminopropyltriethoxysilane modified SiO2 nanoparticles via layer-by-layer electro- static self-assembly. The good spherical shape and size distribution were observed by DLS and TEM analysis. 7-Hydroxycoumarin (7-HC) and rhodamine (RhB) as model drugs were loaded in the core and shell of the nanoparticles seperately. Confocal La- ser Scanning Microscopy (CLSM) shows the core-shell structure of HC-SiO2(PPVA/Cs)n-RhB nanoparticles and the embedded location of 7-HC and RhB. The pH-sensitive release investigation of RhB indicates that the release profiles of RhB from HC-SiO2(PPVA/Cs)3PPVA-RhB core-shell nanoparticles are totally dif- ferent at pH of 2.0, 7.4 and 9.2. These results predict that the multifunctional nanopar- ticle SiO2(PPVA/Cs)n has a great potential for drug delivery. In addition, A new core?shell nanostructure consisting