研究生英文文献汇报系列2课件.pptVIP

5. Structural Image Acquisition Siemens head-only 3T TIM-Trio scanner During unsedated natural sleep 婴儿在自然睡眠状态下的大脑成像 Magnetization prepared rapid acquisition gradient-echo scans were obtained at years 1 and 2 A total of 38 infants at year 1 did not go to sleep or woke up in the scanner (success rate = 57%). A total of 61 infants at year 2 did not go to sleep, woke up in the scanner, or were lost to follow-up (success rate = 31%). * 6. Image Analysis Brain tissue was classified as gray matter, white matter, and cerebrospinal fluid. ITK-SNAP software Obtained measures of intracranial volume, total gray matter, total white matter, total cerebrospinal fluid, lateral ventricle volume, and 90-region gray matter volumes at 1 year of age for 46 subjects and at 2 years of age for 27 subjects. 扫描结果提供了颅内容量、总灰质、总白质、总脑脊液、侧脑室体积和90区域灰质体积等信息 * 7.Association of Cognitive and Brain Outcomes With Cluster Membership and Alpha Diversity Linear mixed effect models were used to test for effects of ： 1. cognitive performance and brain volumes 2. cluster membership on alpha diversity Likelihood ratio tests were used to test the significance of the coefficient of each cluster and alpha diversity measure. Secondary analyses were adjusted for multiple comparisons * 7.Association of Cognitive and Brain Outcomes With Cluster Membership and Alpha Diversity Sensitivity analyses Raw scores T scores Demographic and medical variables associated with cluster membership or alpha diversity and neuroimaging or cognitive outcomes were included as covariates * 8.Metagenome Prediction From PhylogeneticInvestigation of Communities by Reconstructionof Unobserved States PICRUSt （生物信息软件）was used to predict meta-genome functional content from the 16S ribosomal RNA sequencing data. * Results * 1.Infant Microbiota Cluster Into Three Groups Cluster 1 (C1) characterized by a relatively high abundance of Faecalibacterium;第一组的普氏菌属细菌丰度较高 Cluster 2 (C2) by a relatively high abundance of Bacteroides