药剂和工艺半夏干燥过程中褐变机制研究.PDF

中草药 Chinese Traditional and Herbal Drugs 第 42 卷 第 5 期 2011 年 5 月 ·877 · • 药剂与工艺 • 半夏干燥过程中褐变机制的研究 1 2 1 1* 1 严茂伟 ，万 军 ，楚 亮 ，孙灵根 ，吴纯洁 1. 成都中医药大学，四川 成都 611137 2. 西南交通大学，四川 成都 611756 摘 要：目的 研究导致半夏干燥过程中发生褐变的机制。方法 采用紫外分光光度法检测干燥过程中半夏中多酚氧化酶活 性的变化，使用色彩色差计测量半夏表面颜色的变化值，考察酶促反应是否是发生褐变的唯一机制。采用 HPLC-ELSD 法检 测半夏中还原性糖的种类及含量，分析半夏中的氨基酸种类，判断其发生羰氨反应的可能性。结果 导致半夏褐变的反应并 不唯一，酶促反应对其颜色变化的影响主要发生在干燥前期，羰氨反应对半夏褐变也有影响。结论 导致半夏干燥过程中发 生褐变的机制并不单一，酶促反应和羰氨反应均对其颜色变化有影响。 关键词：半夏；颜色变化；褐变机制；多酚氧化酶；羰氨反应；HPLC-ELSD 中图分类号：R283.1 ；R286.02 文献标志码：A 文章编号：0253 - 2670(2011)05 - 0877 - 04 Study on browning mechanism of drying Pinelliae Rhizoma 1 2 1 1 1 YAN Mao-wei , WAN Jun , CHU Liang , SUN Ling-gen , WU Chun-jie 1. Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China 2. Southwest Jiaotong University, Chengdu 611756, China Abstract: Objective To study the browning mechanism of drying Pinelliae Rhizoma . Methods The activity of polyphenol oxidase (PPO) was detected by ultraviolet spectrophotometry, and the surface color data of drying Pinelliae Rhizoma were measured by chromaticity instrument. To draw a conclusion that whether Enzymatic browning was the unique factor of browning reaction or not by analyzing the relationship between the above two sets of data. To detect the kinds and content of reducing sugar by HPLC-ELSD and measure the kinds of amino acids in Pinelliae Rhizoma by amino acid analyzer. Through testing the results the possibility of Maillard reaction was analyzed. Results The factor of the color-change of Pinelliae Rhizoma is not unique, Enzymatic browning is in the prophase of t