嗜酸乳杆菌对螺旋形和球形幽门螺杆菌黏附拮抗作用的研究..doc

嗜酸乳杆菌对螺旋形和球形幽门螺杆菌黏附拮抗作用的研宄 ：吴小茜，佘菲菲，李能，陈月秀 】目的研究热灭活状态和活菌状态嗜酸乳杆菌 对螺旋形和球形幽门螺杆菌(Hp)的黏附拮抗作用。方法制备 人胃上皮腺癌细胞系细胞爬片，将两种状态嗜酸乳杆菌和不 同形态Hp菌分组按不同顺序加入，作用2h，革兰染色后光 镜观察分析Hp黏附指数产生的变化，荧光抗体染色法定性评 价黏附于AGS细胞的Hp密度的改变。结果革兰染色后光镜 观察分析：排除实验组及竞争实验组与对照组比较，黏附指 数均有明显下降。荧光镜下可见实验组黏附于细胞的Hp密 度也明显降低。结论嗜酸乳杆菌对不同形态的Hp均具有黏 附拮抗作用。 关键词】乳杆菌，嗜酸；螺杆菌，幽门；上皮细胞；胃 肿瘤；细菌黏附；荧光抗体技术 TheAntag onismEffectofLacto bacillusAcidophi1u sonAdherenceofHelicalandCoccoidForms sonAdherenceofHeli calandCoccoidForms ofHelicobacterpylo ri WUXiaoqian,SHEF EifEI,LINeng,CHENY uexiu Departmentof PathogenyBiology,F ujianMedicalUniver sity, Fuzhou350004, China ABSTRACT： Ob jective Toinvestig fectonadherenceoft dformsofHptothegas bytheinactivatedan usacidophilus. Metse ededcoverslipswereodifferentstatusof fectonadherenceoft dformsofHptothegas bytheinactivatedan usacidophilus. Met se ededcoverslipswere odifferentstatusof philusandtwodiffer dedindifferentorde acteriawereGarm1ss enceofHptoAGScellw scopeandtheadheren ed. Atthesametime,b yusingFITC tedantibody,theden sityoftheadheredtw pwasevaluatedunder atetheantagonismef hehelicalandcoccoi tricepithelialcell dlivingLactobacill hods TheAGScell prepared,thenthetw Lactobacillusacido entformsofHpweread r. 2hourslater,theb tained,andtheadher ereobservedbymicro ceindexwascalculat conjunc odifferentformsofH thefluoscencemicro twithinactivatedan usacidophilus. Re hecontrolgroup,the thecompetitiontest ontestgroupweremuc tyoftheadheredtwod estgroupsweremuchl scopeaftertreatmen dlivingLactobacill suit Comparedwitht adherenceindexesof groupandtheexclusi hlower,andthedensi ifferentformsHpoft owerthanthatofthec ontrolgroup. Concl vision Lactobacill usacidophilushadan tagonismeffectonce 1 ladherenceofhelic alandcoccoidformso fHp. KEYWORDS: lac tobacillusacidophi lori；epithelialcel s；bacterialadhesiolus；helicobacterpy Is；stomachneoplasm n； f luorescentantib odytechnique lori；epithelialcel s；bacterialadhesio 幽门螺杆菌(Helicobacterpylor i，Hp)存在2种形态： 螺旋野生形和球形变异形。在不利于其生长繁殖的环境中， 螺旋形Hp可转变为球形。球形Hp在细菌的传播及致病中也 起着重要作用