蔬菜和水果中77种有机磷和氨基甲酸酯农药残留量检测技术.PDFVIP

※分析检测 食品科学 2007, Vol. 28, No. 03 247 王明泰，牟 峻，吴 剑，李爱军，卢春梅，周 晓* (吉林出入境检验检疫局技术中心，吉林 长春 130062) ：本方法采用丙酮水溶液(8+2)提取样品，提取液经二氯甲烷液- 液分配、凝胶色谱柱(GPC)净化，固相萃 取柱(活性炭)再净化，浓缩定容后，用气相色谱- 质谱仪(GC-MS)测定，外标法定量。采用选择离子检测进行阳 性确证。选择菠菜、柑橘等10种蔬菜水果为实验样品、敌敌畏等77种农药添加水平在0.05～2.00mg/kg 时，该方 法回收率为 53.6%～124.8%；精密度为4.01%～24.90%；方法测定低限为 0.01～0.200mg/kg。 ：农药残留量；蔬菜及水果；气相色谱- 质谱 Determination Study of 77 Organophosphrous and Carbamate Pesticide Residue in Fruits and Vegetables WANG Ming-tai，MU Jun，WU Jian，LI Ai-jun，LU Chun-mei，ZHOU Xiao* (Technology Center, Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun 130062, China) Abstract ：One testing technology of determining 77 organophosphrous and carbamate pesticides residues in fruits and vegetables is developed. In this study, the samples are extracted with acetone and water (8+2). The extract is distributed with dichloromethane, and then is cleaned by passing through one gel permeation chromatography (GPC) column and one active carbon solid phase extraction (SPE) column in sequence. The eluate is condensed to a definite volume, and then determined and confirmed by gas chromatography - mass spectrometry(GC-MS) with external standard method. Ten vegetables and fruits including spinach and orange etc. are selected as samples. 0.05～2.00mg/kg of the 77 pesticides such as dichlorvos etc. are fortified into blank samples. The corresponding recoveries are 53.6%～124.8% and the precisions are 4.01%～24.90% and the limit of determination (LOD) are 0.01～0.100mg/kg. Key words：pesticide residue；fruits and vegetables；gas chromatography - mass spectrometry(GC-MS) 中图分类号：TS207.53 文献标识码：A 文章编号：1002-6630(2007)03-0247-07 关于水果和蔬菜中多种有机磷