聚酰胺树脂分离纯化神香草提取液工艺的研究.docVIP

聚酰胺树脂分离纯化神香草提取液工艺的研究 　　摘要：目的 考察聚酰胺树脂分离纯化神香草提取液的最佳工艺条件。方法 以总黄酮和迷迭香酸为检测指标，采用单因素考察法筛选树脂纯化工艺中的最大上样量、水洗脱体积、洗脱剂用量、上样液浓度、树脂吸附时间、上样液pH值和洗脱速率等参数。结果 最佳纯化工艺参数为：神香草提取液浓度为10 mg/mL，最大上样量为12 mL，水洗脱2 BV去除杂质，40%乙醇洗脱9 BV，上样液迷迭香酸浓度86.3 μg/mL、总黄酮浓度117.8 μg/mL左右，树脂吸附时间为14 h，上样液pH值调至6.5，洗脱速度3.0 BV/h。结论 该方法简便易行，分离效果良好，适用于神香草提取液的分离纯化。 　　关键词：神香草；聚酰胺树脂；总黄酮；迷迭香酸；纯化工艺 　　DOI：10.3969/j.issn.1005-5304.2017.09. 　　中图分类号：R284.2 文献标识码：A 文章编号：1005-5304（2017）09- 　　Research on Separation and Purification of Extract from Hyssopus cuspidatus Boriss. with Polyamide Resins MAO Yan， HE Jin-hua， WANG Xin-tang， RONG Xiao-juan （Xinjiang Institute of Materia Medica， Urumqi 830004， China） 　　Abstract： Objective To investigate the optimal process conditions for the separation and purification of extract from Hyssopus cuspidatus Boriss. by polyamide resins. Methods The total flavonoids and rosmarinic acid were used as the indexes. The maximum amount of sample solution， elution volume， concentration of sample solution， adsorption time of resin， loading time of sample solution and the amount of eluting solvent， pH and elution rate in the resin purification process were screened by single factor method. Results The optimal purification parameters were as follows： 10 mg/mL of extract， 12 mL of sample amount， 2 BV of water to remove impurities， 40% ethanol to elute 9 BV； the concentration of rosmarinic acid in sample solution was 86.3 μg/mL， and the total flavonoid concentration was 117.8 μg/mL； the resin adsorption time was 14 h； the pH of sample solution was 6.5； the elution rate was 3.0 BV/h. Conclusion This method is simple and feasible， fit for separating and purifying of extract from Hyssopus cuspidatus Boriss. 　　Key words： Hyssopus cuspidatus Boriss.； polyamide resins； total flavonoid； rosmarinic acid； purification process 　　神香草?榇叫慰浦参镉布馍裣悴?Hyssopus cuspidatus Boriss.的干燥地上部分，维吾尔名为“祖发奇尼”，是维吾尔族民间习用药材[1]，性质干热，有很强的香味，具有镇咳、?痰、平喘作用，作为维吾尔医和民间用药已有几百年历史，疗效显著。以神香草为主药的寒喘祖帕颗粒收载于《中华人民共和国卫生部药品标准?维吾尔药分册》1999年版，在新疆维吾尔医院临床