间接竞争化学发光酶免疫法检测动物源食品中的呋喃西林代谢物.PDF

8 2 Vol. 8 No. 2 2017 2 Journal of Food Safety and Quality Feb. , 2017 1, 2 1 1 1 1 3* 黄登宇 , 高丽霞 , 李亚楠 , 冯 敏 , 高文静 , 杨秀松 (1. , 030006; 2. , 030006; 3. , 100073) 摘 要: 目的 方法 (ovalbumin, OVA); , ; 结果 A 8 mmol/L 10 mmol/L (1:1, V:V), B 10 mL () (Tris)-5 µL 30% H2O2 , A B 1:1 ; 800, 1%, 30 min, 60 min; Y=-0.4654X +0.3768(r2=0.993), 0.123~2.398 ng/mL, IC50 0.544 ng/mL, 1.9%~4.1%2.8%~5.3%, 89.6%~98.0%结论 , 关键词: ; ; ; Determination of nitrofurazone metabolite in animal-derived food by indirect competitive chemiluminescence enzyme immunoassay 1,2 1 1 1 1 3* HUANG Deng-Yu , GAO Li-Xia , LI Ya-Nan , FENG Min , GAO Wen-Jing , YANG Xiu-Song (1. College of Life Science, Shanxi University, Taiyuan 030006, China; 2. China Food and Drug Safety Rapid Detection Center , Shanxi University, Taiyuan 030006, China; 3. China Food and Drug Administration Institute of Executive Development , Beijing 100073, China) ABSTRACT: Objective To establish a method for determination