附子生物碱脱毒前后对小菜蛾室内毒力活性比较的研究.docVIP

附子生物碱脱毒前后对小菜蛾室内毒力活性比较的研究 　　摘 要 通过对附子生物碱进行脱毒处理，将双酯型附子生物碱水解为单酯型附子生物碱，分别测定了脱毒前后附子生物碱对小菜蛾的室内毒力活性，包括拒食活性、胃毒活性和生长抑制活性.拒食活性中，非选择性拒食率脱毒前后的毒力回归曲线分别为y=-3.312+2.014X， y=-1.666+1.716X，AFC50分别为0.651和0.971 g/L.选择性拒食率的毒力回归曲线分别为y=-0.859+1.141X，y=-1.718+1.431X，AFC50为0.753和1.201 g/L.胃毒活性中，死亡率的毒力回归曲线分别为y=-1.296+1.838X， y=-1.386+1.379X， LC50分别为0.705和1005 g/L.生长抑制活性中，生长抑制率的毒力回归曲线分别为y=-1.283+1.73X， y=-1.24+1.316X， AGC50分别为0.742和0.942 g/L.分析以上三种活性可知，脱毒前附子生物碱对小菜蛾的杀虫活性主要体现在拒食活性上，其次为生长抑制活性和胃毒活性；脱毒后杀虫活性有所降低，但未全部丧失，尤其在拒食活性上表现明显. 　　关键词 附子生物碱；小菜蛾；毒力回归曲线 　　中图分类号 S435.654文献标识码 A文章编号2016 　　Comparative Study on the Toxicity Activity to Plutella Xylostella by 　　Aconite Alkaloids Both before and after Detoxification in Laboratory 　　BAI Xue1， KANG Xiaohui1*， YANG Suizhuang1， YUAN Xiaohong1， ZHOU Qiang2 　　（1.College of Life Science and Engineering， Southwest University of Science and Technology， Mianyang 621010， China； 　　2.Agricultural Service Center of Yongming Town， Mianyang 621038， China） 　　Abstract Based on the detoxification treatment， making the double ester type of aconite alkaloids hydrolyzed single ester type， we have determined the antifeedant activity， stomach toxicity and growth inhibitory activity by the aconite alkaloids before and after partial detoxification. For the antifeedant activity， the regression curve of the non selective antifeedant rate before and after detoxification is y=-3.312+2.014X， y=-1.666+1.716X， with AFC50 equal to 0.651 and 0.971 g/L， respectively. The regression curve of the selective antifeedant rate is y=-0.859+1.141X， y=-1.718+1.431X， with AFC50 0.753 and 1.201 g/L， respectively. For the gastric toxicity， the regression curve of mortality is y=-1.296+1.838X， y=-1.386+1.379X， with LC50 as 0.705 and 1.005 g/L， respectively. For the growth inhibition activity， the growth inhibition rate of the regression curve is y=-1.283+1.73X， y=-1.24+1.316X， with AGC50 to be 0.742 and 0942 g/L， respectively. It can be seen from above results about the