黄芪―莪术不同提取物抗肿瘤的作用的研究.docVIP

黄芪―莪术不同提取物抗肿瘤的作用的研究 　　[摘要] 目的 研究不同方法提取的?S芪-莪术提取物的抗肿瘤作用。 方法 建立人肝癌细胞株HepG2模型，分别以95%乙醇、50%乙醇、水提醇沉、传统水煎四种方法为提取工艺，用CCK-8法检测不同提取物对HepG2细胞抑制生长作用，并用流式细胞法检测其体外诱导HepG2细胞凋亡的情况。用小鼠肝癌H22细胞建立移植性肝癌模型，检测上述提取物体内抗肿瘤活性，用免疫组织化学法检测H22肝癌组织中NF-κB p65的表达情况。 结果 CCK-8结果显示，与阴性组相比，黄芪-莪术95%乙醇和50%乙醇提取物组最高抑制率分别为89.13%、78.36%，差异有统计学意义（P0.05）。流式细胞术结果显示，95%乙醇提取组和50%乙醇提取物组能明显诱导HepG2细胞凋亡，水提醇沉组和传统水煎组效果不明显。95%乙醇、50%乙醇、水提醇沉、传统水煎四种提取物对小鼠H22荷瘤均有一定的抑瘤效果，抑瘤率分别为47.55%、36.12%、29.96%、34.68%。与模型组相比，95%乙醇和50%乙醇提取物组能明显降低H22荷瘤组织中NF-κB p65水平的表达 （P0.05）。 结论 黄芪-莪术95%乙醇、50%乙醇、水提醇沉、传统水煎四种提取物均有抑制小鼠H22荷瘤生长作用，但是只有95%乙醇和50%乙醇提取物能抑制NF-κB p65蛋白的表达，并有抑制人肝癌HepG2细胞生长及诱导其发生细胞凋亡的作用。 　　[关键词] 黄芪；莪术；HepG2细胞；细胞凋亡；NF-κB p65 　　[中图分类号] R285.5 [文献标识码] B [文章编号] 1673-9701（2017）07-0033-04 　　[Abstract] Objective To study the anti-tumor effect of the extract of Astragalus and Curcuma zedoaria Rosc by different methods. Methods Liver cancer cell line HepG2 model was established by four kinds of methods including 95% ethanol， 50% ethanol， water extraction and ethanol precipitation and the traditional decoction for the extraction process.The inhibitory effect of different extracts on the growth of HepG2 cells was detected by CCK-8 method.And the apoptosis of HepG2 cells was detected by flow cytometry.The model of transplanted hepatocellular carcinoma（HCC） was established by mouse liver cancer H22 cells.The antitumor activity in the above-mentioned extract was examined. The expression of NF-κB p65 in H22 hepatocellular carcinoma tissues was detected by immunohistochemistry. Results The CCK-8 results showed that the highest inhibitory rates were 89.13% and 78.36% in Astragalus-Curcuma 95% ethanol and 50% ethanol extraction groups respectively compared with that in the negative group， and the difference was significant （P0.05）.The results of flow cytometry showed that 95% ethanol extraction group and 50% ethanol extraction group could obviously induce HepG2 cell apoptosis， while the roles of the water extraction and alcohol precipitation group