高色价栀子黄精制工艺研究.PDFVIP

高色价栀子黄的精制工艺研究  刘慧璐，冯建勇，王增尚，吴晨，张末初，周胜，刘江云* ，郝丽莉(苏州大学药学院，江苏 苏州 215123) 摘要：目的 对栀子黄进行进一步分离制备，以获得高色价的西红花总苷及其中主要单体成分。方法 采用中压反相柱 层析技术，对栀子黄进行进一步分离制备，采用液相色谱检测西红花苷-1 含量，采用紫外可见光谱检测色价及其吸光度 比值。结果 制备工艺可有效去除绿原酸等杂质，精制后的西红花总苷样品中西红花苷-1 含量为60.8% ，色价高达756 ； 并可分离获得西红花苷-1(1) 、西红花苷-2(2) 、西红花苷-3(3)等化合物。结论 该制备方法简便、高效、成本较低，可为 栀子黄的精制及产业化生产提供参考。 关键词：栀子；栀子黄；西红花苷；分离；反相柱层析 中图分类号：R284.1 ；R917.101 文献标志码：A 文章编号：1007-7693(2013)12-1315-05 Preparative Separation of Crocins with High Color Value from Gardenia Fruits * LIU Huilu, FENG Jianyong, WANG Zengshang, WU Chen, ZHANG Mochu, ZHOU Sheng, LIU Jiangyun , HAO Lili(College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China) ABSTRACT: OBJECTIVE To establish an efficient separation process to afford gardenia yellow and crocins with high color value. METHODS The process was conducted using reversed-phase medium pressure liquid chromatography. A high pressure liquid chromatography - photodiode array method was applied for qualitative and quantitative analysis of crocin-1. Moreover, the color value was also evaluated by UV method. RESULTS After the reversed-phase separation, the content of crocin-1 in refined product reached 60.8%, with the color value to be 756. Crocin-1(1), crocin-2(2) and crocin-3(3) could be separated either with similar procedure. CONCLUSION The process developed is highly efficient, low-cost and compact, which affords a potential approach for industrial production of crocins with improved quality. KEY WORDS: Gardenia jasminoides; gardenia yellow; crocin; separation; reversed-phase chromatography 西红花苷(藏红花素) 是一类珍贵的水溶性胡 栀子黄制备工艺的相关研究主要集中在我 萝卜素衍生物，是名贵药材藏红花中的主要有效 国，涉及溶剂提取方法、萃取技术、树脂精制、 成分，具有抗氧化、抗肿瘤等多种功效，还作为 膜分离技