BOLD+fMRI技术结合非血流动力学响应函数分析法检测大鼠C6皮下胶质瘤内部氧合变化的可行性-研究.pdf

Abstract BOLD fMRI 技术结合非血流动力学响应函数分析法检测大鼠C6 皮下胶质瘤内部氧合变化的可行性研究 The Feasibility Study of Using BOLD fMRI with Non-hemodynamic Response Function Analysis Method to detect the oxygenation changes within rats subcutanous C6 glioma Abstract Part I Animal Model Establishment and Pathological Observation of rats bearing C6 subcutanous glioma Objectives: Compare the tumor formation rate and growth rule of the C6 subcutanous glioma models established by C6 cell suspension inoculation and tumor mass inoculation methods respectively,and select the optimal method for the second part of the experiment. Methods: 21 Wistar rats were divided into two groups including C6 cell suspension inoculation group and tumor mass inoculation group to establish the tumor models. The formation rate, latent period, diameters and relative volume of the tumor were observed and recorded regularly. Compare the differences of the formation rate,latent period and the maximum diameter and the relative volume of the tumor between the two groups at different time points after inoculation,and analysis the growth tendency of the tumor. Compare the differences of the formation rate and latent period between the two groups. All of tumors were perform HE staining with the tumor paraffin section and observe the pathological features by microscope. Results: 1. The tumor formation rate of the cell suspension inoculation group was 100%, and the latency was 8.45±0.69(d), while another group was 60% and 9.83±1.72(d). 2. The maximum diameter of the tumors estabilished by cell suspension inoculation were much bigger than the ones built by tumor mass inoculation during 14days to 20days after inoculation.Similarly, there were significant statistically differences